Morphological characteristics of young and old murine hematopoietic stem cell niches, as modeled in vitro

Justin, Mojca (avtor), Rogač Randl, Ema (avtor), Kononenko, Veno (avtor), Hočevar, Matej (avtor), Drobne, Damjana (avtor), Rožman, Primož (avtor)

The hematopoietic stem cell (HSC) niche undergoes detrimental changes with age. The molecular differences between young and old niches are well studied and understood; however, young and old niches have not yet been extensively characterized in terms of morphology. In the present work, a 2D stromal model of young and old HSC niches isolated from bone marrow was investigated using light and scanning electron microscopy (SEM) to characterize cell density after one, two, or three weeks of culturing, cell shape, and cell surface morphological features. Our work is aimed at identifying morphological differences between young and old niche cells that could be used to discriminate between their respective murine HSC niches. The results show several age-specific morphological characteristics. The old niches differ from the young ones in terms of lower cell proliferating capacity, increased cell size with a flattened appearance, increased number of adipocytes, and the presence of tunneling nanotubes. In addition, proliferating cell clusters are present in the young niches but not in the old niches. Together, these characteristics could be used as a relatively simple and reliable tool to discriminate between young and old murine HSC niches and as a complementary approach to imaging with specific cellular markers.

• Jezik: Angleški jezik
• Ključne besede: bone marrow, hematopoietic stem cell niches, aging, adipocytes, scanning electron microscopy
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: 10 str.
• Številčenje: Vol. 2023, art. 5541050
• PID: 20.500.12556/RUL-152339
• UDK: 576
• ISSN pri članku: 1932-8745
• DOI: 10.1155/2023/5541050
• COBISS.SI-ID: 149390083
• Datum objave v RUL: 21.11.2023

Gradivo je del revije

• Naslov: Scanning
• Založnik: Hindawi
• ISSN: 1932-8745
• COBISS.SI-ID: 517825049

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: kostni mozeg, niše hematopoetskih matičnih celic, staranje, adipociti, vrstična elektronska mikroskopija