Eye and hair color prediction of an early medieval adult and subadult skeleton using massive parallel sequencing technology

Zupanič-Pajnič, Irena (avtor), Leskovar, Tamara (avtor), Črešnar, Matija (avtor)

Phenotypic trait prediction in ancient DNA analysis can provide information about the external appearance of individuals from past human populations. Some studies predicting eye and hair color in ancient adult skeletons have been published, but not for ancient subadult skeletons, which are more prone to decay. In this study, eye and hair color were predicted for an early medieval adult skeleton and a subadult skeleton that was anthropologically characterized as a middle-aged man and a subadult of unknown sex about 6 years old. When processing the petrous bones, precautions were taken to prevent contamination with modern DNA. The MillMix tissue homogenizer was used for grinding, 0.5 g of bone powder was decalcified, and DNA was purified in Biorobot EZ1. The PowerQuant System was used for quantification and a customized version of the HIrisPlex panel for massive parallel sequencing (MPS) analysis. Library preparation and templating were performed on the HID Ion Chef Instrument and sequencing on the Ion GeneStudio S5 System. Up to 21 ng DNA/g of powder was obtained from ancient petrous bones. Clean negative controls and no matches with elimination database profiles confirmed no contamination issue. Brown eyes and dark brown or black hair were predicted for the adult skeleton and blue eyes and brown or dark brown hair for the subadult skeleton. The MPS analysis results obtained proved that it is possible to predict hair and eye color not only for an adult from the Early Middle Ages, but also for a subadult skeleton dating to this period.

• Jezik: Angleški jezik
• Ključne besede: skeletal remains, ancient DNA, petrous bones, eye color, hair color, HIrisPlex
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta ; FF - Filozofska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: Str. 1629–1638
• Številčenje: Vol. 137, iss. 5
• PID: 20.500.12556/RUL-151968
• UDK: 61
• ISSN pri članku: 1437-1596
• DOI: 10.1007/s00414-023-03032-y
• COBISS.SI-ID: 154897411
• Datum objave v RUL: 26.10.2023

Gradivo je del revije

• Naslov: International journal of legal medicine
• Skrajšan naslov: Int. j. leg. med.
• Založnik: Springer Nature, International Academy of Legal Medicine
• ISSN: 1437-1596
• COBISS.SI-ID: 512020249

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: skeletni ostanki, starodavna DNK, petrous kosti

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: J3-3080
• Naslov: Določitev predniškega porekla iz DNA za identifikacijske namene