Nanofibers with genotyped Bacillus strains exhibiting antibacterial and immunomodulatory activity

Grilc, Nina Katarina (avtor), Zidar, Anže (avtor), Kocbek, Petra (avtor), Rijavec, Tomaž (avtor), Colja, Teja (avtor), Lapanje, Aleš (avtor), Jeras, Matjaž (avtor), Gobec, Martina (avtor), Mlinarič-Raščan, Irena (avtor), Gašperlin, Mirjana (avtor), Kristl, Julijana (avtor), Zupančič, Špela (avtor)

Biofilm-associated diseases such as periodontitis are widespread and challenging to treat which calls for new strategies for their effective management. Probiotics represent a promising approach for targeted treatment of dysbiosis in biofilm and modulation of host immune response. In this interdisciplinary study, nanofibers with two autochthonous Bacillus strains 27.3.Z and 25.2.M were developed. The strains were isolated from the oral microbiota of healthy individuals, and their genomes were sequenced and screened for genes associated with antimicrobial and immunomodulatory activities, virulence factors, and transferability of resistance to antibiotics. Spores of two Bacillus strains were incorporated individually or in combination into hydrophilic poly(ethylene oxide) (PEO) and composite PEO/alginate nanofibers. The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature. Spores were rapidly released from PEO nanofibers, while presence of alginate in the nanofibers prolonged their release. All formulations exhibited swelling, followed by transformation of the nanofiber mat into a hydrogel and polymer erosion mediating spore release kinetics. The investigated Bacillus strains released metabolites, which were not cytotoxic to peripheral blood mononuclear cells (PBMCs) in vitro. Moreover, their metabolites exhibited antibacterial activity against two periodontopathogens, an antiproliferative effect on PBMCs, and inhibition of PBMC expression of proinflammatory cytokines. In summary, the developed nanofiber-based delivery system represents a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites.

• Jezik: Angleški jezik
• Ključne besede: probiotics, biofilm-related disease, periodontal disease, bacteria encapsulation, electrospinning, viability, release, erosion
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FFA - Fakulteta za farmacijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: Str. 371-384
• Številčenje: Vol. 355
• PID: 20.500.12556/RUL-148322-fbc49374-2873-e3d6-0d2c-901b44a9d7c9
• UDK: 66.017-022.513.2:616.311.2
• ISSN pri članku: 0168-3659
• DOI: 10.1016/j.jconrel.2023.01.082
• COBISS.SI-ID: 141431811
• Datum objave v RUL: 11.08.2023

Gradivo je del revije

• Naslov: Journal of controlled release
• Skrajšan naslov: J. control. release
• Založnik: Elsevier
• ISSN: 0168-3659
• COBISS.SI-ID: 27318784

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: z biofilmom povezane bolezni, inkapsulacija bakterij, elektropredenje, preživetje, sproščanje, erozija, nanovlakna, probiotiki, parodontalne bolezni