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Production of aflatoxin B1 by Aspergillus parasiticus grown on a novel meat-based media
ID Zahija, Iva (Avtor), ID Jeršek, Barbara (Avtor), ID Demšar, Lea (Avtor), ID Lušnic Polak, Mateja (Avtor), ID Polak, Tomaž (Avtor)

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Izvleček
The aim of the present study was to develop meat-based media with compositions similar to those of dry-fermented meat products and to evaluate their use in studying the growth of Aspergillus parasiticus and the kinetics of aflatoxin B1 (AFB1) production. In our previous experiments, we found that the strain A. parasiticus ŽMJ7 produced a high amount of AFB1. Cooked meat agar (CMA2) was used as a novel complex meat-based medium with four variations: CMA2G (CMA2 supplemented with 1% glucose), CMA2YE (CMA2 supplemented with 0.2% yeast extract), and CMA2GYE (CMA2 supplemented with 1% glucose and 0.2% yeast extract). Media were inoculated with an A. parasiticus spore suspension (105 spores/mL) and incubated at 25 °C for up to 15 days. The A. parasiticus lag phase lasted less than 1 day, irrespective of the growth medium, with the exception of control medium CMA1 (cooked meat agar) as an already known meat-based medium. The highest mean colony growth rate was observed on CMA2 and CMA2G. Reversed-phase UPLC–MS/MS analysis was performed to determine the AFB1 concentration in combination with solid phase extraction (SPE). The highest AFB1 concentration in meat-based media was detected in CMA2GYE after 15 days of incubation (13,502 ± 2367 ng/mL media). The results showed that for studying AFB1 production in dry-fermented meat products, novel suitable media such as CMA2-based media are required. This finding could represent a potential concern with regard to the production of dry-fermented meat products.

Jezik:Angleški jezik
Ključne besede:moulds, Aspergillus parasiticus, mycotoxins, aflatoxin B1, meat-based media
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:BF - Biotehniška fakulteta
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2023
Št. strani:17 str.
Številčenje:Vol. 15, iss. 1
PID:20.500.12556/RUL-145897 Povezava se odpre v novem oknu
UDK:579.24:615.9:582.282.123.4
ISSN pri članku:2072-6651
DOI:10.3390/toxins15010025 Povezava se odpre v novem oknu
COBISS.SI-ID:135836163 Povezava se odpre v novem oknu
Datum objave v RUL:17.05.2023
Število ogledov:642
Število prenosov:74
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
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Gradivo je del revije

Naslov:Toxins. Elektronski vir
Skrajšan naslov:Toxins
Založnik:MDPI
ISSN:2072-6651
COBISS.SI-ID:517594649 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:plesni, mikotoksini, aflatoksin B1, mikrobiološka gojišča

Projekti

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P4-0234
Naslov:Integrirano živilstvo in prehrana

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