Estrogenic potency of endocrine disrupting chemicals and their mixtures detected in environmental waters and wastewaters

Durcik, Martina (avtor), Grobin, Andrej (avtor), Roškar, Robert (avtor), Trontelj, Jurij (avtor), Peterlin-Mašič, Lucija (avtor)

Endocrine disrupting chemicals such as natural and synthetic steroid hormones and bisphenols are among the most important pollutants in the aquatic environment. We performed an environmental chemical analysis of five Slovenian water samples, two rivers, one groundwater, and the influent and effluent of wastewater treatment plants, with a highly sensitive analysis of twenty-five endocrine-disrupting compounds belonging to the groups of natural hormones, synthetic hormones, and bisphenols. Since these compounds are simultaneously present in the environment, it is important to study their individual effects as well as the effects of mixtures. We investigated in vitro the estrogenic potency of selected natural and synthetic steroid hormones and bisphenols detected in surface, ground and waste water in Slovenia using the OECD-validated transactivation assay on the cell line Hela9903. We predicted their mixture effects using the concentration addition model and compared them with experimentally determined values. Two mixing designs were used: a balanced design in which chemicals were combined in proportion to their individual EC$_{50}$ values, and an unbalanced design with compounds in proportion to their measured concentrations in the environmental samples. The estrogenic effects of the experimental mixtures followed the concentration addition model. Real water samples exhibited weaker estrogenic effects, showing the great heterogeneity of the real water samples.

• Jezik: Angleški jezik
• Ključne besede: bisphenols, concentration addition, environmental water, estrogenic activity, hormones, mixtures
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FFA - Fakulteta za farmacijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: 13 str.
• Številčenje: Vol. 330, art. 138712
• PID: 20.500.12556/RUL-145648
• UDK: 612.43
• ISSN pri članku: 0045-6535
• DOI: 10.1016/j.chemosphere.2023.138712
• COBISS.SI-ID: 150677507
• Datum objave v RUL: 04.05.2023

Gradivo je del revije

• Naslov: Chemosphere
• Skrajšan naslov: Chemosphere
• Založnik: Pergamon Press.
• ISSN: 0045-6535
• COBISS.SI-ID: 25213696

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: bisfenoli, dodatek koncentracij, okoljska voda, estrogena aktivnost, hormoni, mešanice, hormonski motilci, estrogeni

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P1-0208
• Naslov: Farmacevtska kemija: načrtovanje, sinteza in vrednotenje učinkovin

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P1-0189
• Naslov: Farmacevtska tehnologija: od dostavnih sistemov učinkovin do terapijskih izidov zdravil pri otrocih in starostnikih