Single cell RNA sequencing in autoimmune inflammatory rheumatic diseases : current applications, challenges and a step toward precision medicine

Kuret, Tadeja (avtor), Sodin-Šemrl, Snežna (avtor), Leskošek, Branimir (avtor), Ferk, Polonca (avtor)

Single cell RNA sequencing (scRNA-seq) represents a new large scale and high throughput technique allowing analysis of the whole transcriptome at the resolution of an individual cell. It has emerged as an imperative method in life science research, uncovering complex cellular networks and providing indices that will eventually lead to the development of more targeted and personalized therapies. The importance of scRNA-seq has been particularly highlighted through the analysis of complex biological systems, in which cellular heterogeneity is a key aspect, such as the immune system. Autoimmune inflammatory rheumatic diseases represent a group of disorders, associated with a dysregulated immune system and high patient heterogeneity in both pathophysiological and clinical aspects. This complicates the complete understanding of underlying pathological mechanisms, associated with limited therapeutic options available and their long-term inefficiency and even toxicity. There is an unmet need to investigate, in depth, the cellular and molecular mechanisms driving the pathogenesis of rheumatic diseases and drug resistance, identify novel therapeutic targets, as well as make a step forward in using stratified and informed therapeutic decisions, which could now be achieved with the use of single cell approaches. This review summarizes the current use of scRNA-seq in studying different rheumatic diseases, based on recent findings from published in vitro, in vivo, and clinical studies, as well as discusses the potential implementation of scRNA-seq in the development of precision medicine in rheumatology.

• Jezik: Angleški jezik
• Ključne besede: rheumatoid arthritis, systemic lupus erythematosus, RNA sequencing, single cell, systemic sclerosis, precision medicine
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.02 - Pregledni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2022
• Št. strani: 18 str.
• Številčenje: Vol. 8, art. 822804
• PID: 20.500.12556/RUL-145362
• UDK: 616-002
• ISSN pri članku: 2296-858X
• DOI: 10.3389/fmed.2021.822804
• COBISS.SI-ID: 93835779
• Datum objave v RUL: 19.04.2023

Gradivo je del revije

• Naslov: Frontiers in medicine
• Skrajšan naslov: Front. med.
• Založnik: Frontiers Media
• ISSN: 2296-858X
• COBISS.SI-ID: 523095065

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: revmatoidni artritis, sistemski eritematozni lupus, sekvenciranje RNA

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P3-0154
• Naslov: Metodologija za analizo podatkov v medicini