Systems view of Bacillus subtilis pellicle development

Krajnc, Mojca (avtor), Štefanič, Polonca (avtor), Kostanjšek, Rok (avtor), Mandić-Mulec, Ines (avtor), Dogša, Iztok (avtor), Stopar, David (avtor)

In this study, we link pellicle development at the water–air interface with the vertical distribution and viability of the individual B. subtilis PS-216 cells throughout the water column. Real-time interfacial rheology and time-lapse confocal laser scanning microscopy were combined to correlate mechanical properties with morphological changes (aggregation status, filament formation, pellicle thickness, spore formation) of the growing pellicle. Six key events were identified in B. subtilis pellicle formation that are accompanied by a major change in viscoelastic and morphology behaviour of the pellicle. The results imply that pellicle development is a multifaceted response to a changing environment induced by bacterial growth that causes population redistribution within the model system, reduction of the viable habitat to the water–air interface, cell development, and morphogenesis. The outcome is a build-up of mechanical stress supporting structure that eventually, due to nutrient deprivation, reaches the finite thickness. After prolonged incubation, the formed pellicle collapses, which correlates with the spore releasing process. The pellicle loses the ability to support mechanical stress, which marks the end of the pellicle life cycle and entry of the system into the dormant state.

• Jezik: Angleški jezik
• Ključne besede: Bacillus subtilis, biofilm, cell interactions, bacteriology
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2022
• Št. strani: 11 str.
• Številčenje: Vol. 8, art. 25
• PID: 20.500.12556/RUL-145263
• UDK: 579.22/.24:579.852.11
• ISSN pri članku: 2055-5008
• DOI: 10.1038/s41522-022-00293-0
• COBISS.SI-ID: 105098243
• Datum objave v RUL: 14.04.2023

Gradivo je del revije

• Naslov: npj biofilms and microbiomes
• Skrajšan naslov: npj biofilms microbiomes
• Založnik: Springer Nature, Nanyang Technological University
• ISSN: 2055-5008
• COBISS.SI-ID: 525632025

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: Bacillus subtilis, biofilm, celične interakcije