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Novel identification of the collection of pathogenic fungal species Verticillium with the development of species-specific SSR markers
ID
Jeseničnik, Taja
(
Avtor
),
ID
Kaurin, Anela
(
Avtor
),
ID
Grgič, Zarja
(
Avtor
),
ID
Radišek, Sebastjan
(
Avtor
),
ID
Jakše, Jernej
(
Avtor
),
ID
Štajner, Nataša
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(2,87 MB)
MD5: 6460E72F84706419A6A0C82B315D260F
URL - Izvorni URL, za dostop obiščite
https://www.mdpi.com/2076-0817/12/4/535
Galerija slik
Izvleček
The genus Verticillium is a group of ascomycete fungi that includes several pathogenic plant species. In 2011, a new taxonomic classification, proposed by Inderbitzin and coworkers (2011), re-defined the genus as Verticillium sensu stricto. The objective of our study was the re-classification of the fungal species held in the culture collection in the Slovenian Institute of Hop Research and Brewing in accordance with the newly established taxonomy. With the PCR marker system proposed by Inderbitzin and coworkers in 2011, we re-classified 88 Verticillium isolates out of the 105 samples that are held in the institute’s bank, which were obtained from different geographic locations in Europe, North America, and Japan, and from different host plants, including alfalfa, cotton, hop, olive, potato, and tomato. However, the PCR marker for the V. dahliae identification proved to be less specific, and it resulted in the positive amplification of Gibellulopsis nigrescens, V. isaacii, and V. longisporum. To enable the accurate distinction of the fungi, the SSR and LAMP markers were added to the analyses. The 12 newly identified SSR markers, which were used in simplex PCR reactions or in combination, enabled the accurate identification of all included Verticillium isolates and could potentially be used as biomarkers for rapid and easy species identification.
Jezik:
Angleški jezik
Ključne besede:
classification
,
LAMP
,
multiplex PCR
,
SSR marker
,
Vericillium
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
BF - Biotehniška fakulteta
Leto izida:
2023
Št. strani:
18 str.
Številčenje:
Vol. 12, no. 4, art. 535
PID:
20.500.12556/RUL-145021
UDK:
577.2
ISSN pri članku:
2076-0817
COBISS.SI-ID:
147144451
Datum objave v RUL:
30.03.2023
Število ogledov:
423
Število prenosov:
59
Metapodatki:
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Objavi na:
Gradivo je del revije
Naslov:
Pathogens
Skrajšan naslov:
Pathogens
Založnik:
MDPI
ISSN:
2076-0817
COBISS.SI-ID:
523338009
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
glive
,
Verticillium
,
identifikacija
,
klasifikacija
,
SSR markerji
,
PCR
Projekti
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Program financ.:
Young Researchers
Številka projekta:
34110
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P4-0077
Naslov:
Kmetijske rastline - genetika in sodobne tehnologije
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