Vaš brskalnik ne omogoča JavaScript!
JavaScript je nujen za pravilno delovanje teh spletnih strani. Omogočite JavaScript ali pa uporabite sodobnejši brskalnik.
Nacionalni portal odprte znanosti
Odprta znanost
DiKUL
slv
|
eng
Iskanje
Brskanje
Novo v RUL
Kaj je RUL
V številkah
Pomoč
Prijava
Detrimental effect of various preparations of the human amniotic membrane homogenate on the 2D and 3D bladder cancer in vitro models
ID
Janev, Aleksandar
(
Avtor
),
ID
Železnik Ramuta, Taja
(
Avtor
),
ID
Tratnjek, Larisa
(
Avtor
),
ID
Sardoč, Žiga
(
Avtor
),
ID
Obradović, Hristina
(
Avtor
),
ID
Mojsilović, Slavko
(
Avtor
),
ID
Taskovska, Milena
(
Avtor
),
ID
Smrkolj, Tomaž
(
Avtor
),
ID
Erdani-Kreft, Mateja
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(13,78 MB)
MD5: 8DA335C81329B4AB3D0FE859E9E5EA45
URL - Izvorni URL, za dostop obiščite
https://www.frontiersin.org/articles/10.3389/fbioe.2021.690358/full
Galerija slik
Izvleček
Despite being among the ten most common cancers with high recurrence rates worldwide, there have been no major breakthroughs in the standard treatment options for bladder cancer in recent years. The use of a human amniotic membrane (hAM) to treat cancer is one of the promising ideas that have emerged in recent years. This study aimed to investigate the anticancer activity of hAM homogenate on 2D and 3D cancer models. We evaluated the effects of hAM homogenates on the human muscle invasive bladder cancer urothelial (T24) cells, papillary cancer urothelial (RT4) cells and normal porcine urothelial (NPU) cells as well as on human mammary gland nontumorigenic (MCF10a) cells and low-metastatic breast cancer (MCF7) cells. After 24 h, we observed a gradual detachment of cancerous cells from the culture surface, while the hAM homogenate did not affect the normal cells. The most pronounced effect hAM homogenate had on bladder cancer cells; however, the potency of their detachment was dependent on the treatment protocol and the preparation of hAM homogenate. We demonstrated that hAM homogenate significantly decreased the adhesion, growth, and proliferation of human bladder invasive and papillary cancer urothelial cells and did not affect normal urothelial cells even in 7-day treatment. By using light and electron microscopy we showed that hAM homogenate disrupted the architecture of 2D and 3D bladder cancer models. The information provided by our study highlights the detrimental effect of hAM homogenate on bladder cancer cells and strengthens the idea of the potential clinical application of hAM for bladder cancer treatment.
Jezik:
Angleški jezik
Ključne besede:
cancer
,
urothelium
,
2D and 3D in vitro models
,
light and electron microscopy
,
proliferation
,
cell cycle
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
MF - Medicinska fakulteta
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2021
Št. strani:
19 str.
Številčenje:
Vol. 9, art. 690358
PID:
20.500.12556/RUL-144672
UDK:
616.6
ISSN pri članku:
2296-4185
DOI:
10.3389/fbioe.2021.690358
COBISS.SI-ID:
69118467
Datum objave v RUL:
07.03.2023
Število ogledov:
1123
Število prenosov:
52
Metapodatki:
Citiraj gradivo
Navadno besedilo
BibTeX
EndNote XML
EndNote/Refer
RIS
ABNT
ACM Ref
AMA
APA
Chicago 17th Author-Date
Harvard
IEEE
ISO 690
MLA
Vancouver
:
Kopiraj citat
Objavi na:
Gradivo je del revije
Naslov:
Frontiers in bioengineering and biotechnology
Skrajšan naslov:
Front. bioeng. biotechnol.
Založnik:
Frontiers Media
ISSN:
2296-4185
COBISS.SI-ID:
523093017
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
rak sečnega mehurja
,
urotelij
,
2D in 3D in vitro modeli
,
elektronski mikroskop
,
proliferacija
,
urologija
Projekti
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Program financ.:
Young researchers
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
J7-2594
Naslov:
Uporaba amnijske membrane za inovativno multimodalno zdravljenje bakterijskega cistitisa in raka sečnega mehurja: učinek njene protimikrobne, imunomodulatorne in protirakave aktivnosti
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P3-0108
Naslov:
Diferenciacija urotelijskih celic
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
I0-0022
Naslov:
Mreža raziskovalnih infrastrukturnih centrov Univerze v Ljubljani (MRIC UL)
Podobna dela
Podobna dela v RUL:
Podobna dela v drugih slovenskih zbirkah:
Nazaj