Bacterial DNA recognition by SERS active plasma-coupled nanogold

Shvalya, Vasyl (avtor), Skubic, Cene (avtor), Nadižar, Nejc (avtor), Rozman, Damjana (avtor), Cvelbar, Uroš (avtor)

It is shown that surface-enhanced Raman spectroscopy (SERS) can identify bacteria based on their genomic DNA composition, acting as a “sample-distinguishing marker”. Successful spectral differentiation of bacterial species was accomplished with nanogold aggregates synthesized through single-step plasma reduction of the ionic gold-containing vapored precursor. A high enhancement factor (EF = 10$^7$) in truncated coupled plasmonic particulates allowed SERS-probing at nanogram sample quantities. Simulations confirmed the occurrence of the strongest electric field confinement within nanometric gaps between gold dimers/chains from where the molecular fingerprints of bacterial DNA fragments gained photon scattering enhancement. The most prominent Raman modes linked to fundamental base-pair molecular vibrations were deconvoluted and used to proceed with nitrogenous base content estimation. The genomic composition (percentage of guanine-cytosine and adenine-thymine) was successfully validated by third-generation sequencing using nanopore technology, further proving that the SERS technique can be employed to swiftly specify bioentities by the discriminative principal-component statistical approach.

• Jezik: Angleški jezik
• Ključne besede: DNA genomic ration, plasma electrochemical reduction, coupled plasmonic nanogold, DNA Raman fingerprints, genetics, genomics, gold, plasma, Raman spectroscopy
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2022
• Št. strani: Str. 9757-9765
• Številčenje: Vol. 22, iss. 23
• PID: 20.500.12556/RUL-144312
• UDK: 533
• ISSN pri članku: 1530-6992
• DOI: 10.1021/acs.nanolett.2c02835
• COBISS.SI-ID: 127669763
• Datum objave v RUL: 14.02.2023

Gradivo je del revije

• Naslov: Nano letters
• Skrajšan naslov: Nano lett.
• Založnik: American Chemical Society
• ISSN: 1530-6992
• COBISS.SI-ID: 512808217

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.