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Preučevanje odpornosti krompirja (Solanum tuberosum) proti krompirjevi plesni (Phytophthora infestans), posredovane z genom Rpi-Smira2
ID Blatnik, Eva (Avtor), ID Meglič, Vladimir (Mentor) Več o mentorju... Povezava se odpre v novem oknu

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Izvleček
Krompirjevo plesen povzroča oomiceta Phytophthora infestans, ki močno ogroža globalno pridelavo krompirja. Sorta Sárpo Mira velja za eno izmed bolj odpornih krompirjevih sort proti P. infestans, h kateri naj bi najbolj prispeval gen Rpi-Smira2/R8. Iz populacije F1 križancev, med petimi občutljivimi sortami in sorto Sárpo Mira, smo z genskimi markerji in efektorsko agroinfiltracijo izbrali deset genotipov R8, ki so vsebovali le gen Rpi-Smira2/R8, in jih izpostavili štirim različno agresivnim izolatom P. infestans, da pri preučili doprinos gena Rpi-Smira2/R8 k stopnji odpornosti proti krompirjevi plesni. Genotipe R8 smo, skupaj s pripadajočimi starševskimi sortami, inokulirali z izolati P. infestans na ravni tkivnih kultur in na ravni celih rastlin v rastlinjaku. Izmed testiranih genotipov R8 je genotip C571 izražal najvišjo stopnjo odpornosti, ki je bila po inokulaciji s tujimi izolati P. infestans primerljiva s starševsko sorto Sárpo Mira. V primeru genotipa C571 se je stopnja odpornosti ohranila tako pri inokulaciji tkivnih kultur kot tudi pri inokulaciji celih rastlin v rastlinjaku, medtem ko je bila sorta Sárpo Mira na ravni tkivnih kultur srednje odporna proti agresivnim tujim izolatom, na ravni celih rastlin v rastlinjaku pa je bila nanje popolnoma odporna. Z raziskavami v okviru doktorske disertacije smo tako pokazali, da gen Rpi-Smira2/R8 doprinese k stopnji odpornosti proti krompirjevi plesni, vendar je le-ta nižja v primerjavi s sorto Sárpo Mira in je pod vplivom genetskega ozadja.

Jezik:Slovenski jezik
Ključne besede:krompir, krompirjeva plesen, Phytophthora infestans, gen Rpi-Smira2/R8, Sárpo Mira, genotipi R8, genski markerji, stopnja odpornosti, AUDPC, rastlinske tkivne kulture
Vrsta gradiva:Doktorsko delo/naloga
Tipologija:2.08 - Doktorska disertacija
Organizacija:BF - Biotehniška fakulteta
Leto izida:2023
PID:20.500.12556/RUL-143907 Povezava se odpre v novem oknu
COBISS.SI-ID:138786563 Povezava se odpre v novem oknu
Datum objave v RUL:19.01.2023
Število ogledov:1282
Število prenosov:115
Metapodatki:XML DC-XML DC-RDF
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Sekundarni jezik

Jezik:Angleški jezik
Naslov:Study of the late blight (Phytophthora infestans) resistance in potato (Solanum tuberosum) mediated by gene Rpi-Smira2
Izvleček:
Late blight is caused by the oomycete Phytophthora infestans and is a major threat to the global potato production. Sárpo Mira cultivar is considered to be highly resistant to P. infestans, to which the Rpi-Smira2/R8 gene is said to contribute the most. From the population of F1 progeny, between five susceptible cultivars and Sárpo Mira cultivar, we selected ten R8 genotypes containing only the Rpi-Smira2/R8 gene using genetic markers and effector agroinfiltration, and exposed them to four P. infestans isolates differing in their aggressiveness, to evaluate the contribution of the Rpi-Smira2/R8 gene to the late blight resistance level. R8 genotypes, together with the corresponding parental varieties, were inoculated with P. infestans isolates as tissue cultures and as whole plants in the greenhouse. Among the tested R8 genotypes, the highest level of resistance was shown by genotype C571, which was comparable to the parental variety Sárpo Mira after inoculation with foreign isolates of P. infestans. In the case of genotype C571, the level of resistance was maintained both in tissue culture inoculation and in inoculation of whole plants in the greenhouse, while the Sárpo Mira cultivar was moderately resistant to aggressive foreign isolates at the level of tissue cultures and completely resistant as a whole plant in the greenhouse. With the research conducted in the scope of the doctoral dissertation, we have shown that the Rpi-Smira2/R8 gene contributes to late blight resistance, however the level of resistance is lower compared to the Sárpo Mira cultivar and under influence of the genetic background.

Ključne besede:potato, late blight, Phytophthora infestans, Rpi-Smira2/R8 gene, Sárpo Mira, R8 genotypes, genetic markers, resistance level, AUDPC, plant tissue culture

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