Due to the rapid synthesis in the production of many new chemicals, there is a need to test their toxicity. Large quantities are used in toxicity testing and therefore a lot of waste chemicals are also produced during testing. Therefore, the aim of the thesis was the miniaturization of the standard ecotoxicity test with duckweed Lemna minor. The results of the standard and miniaturized test (in microtiter plates of different volumes) were compared with each other, and thus the appropriate miniaturization of the test was determined. KCl solution was used as the standard substance and the results of inhibition of specific growth rate and root length were compared. The most appropriate miniaturized system was used to test the toxicity of zinc oxide nanoparticles. The nanoparticles were toxic to duckweed, and additional testing revealed that the toxicity was due to the dissolution of zinc from the nanoparticles. A comparison of the toxicity test results in the beakers and the miniaturized system showed a good fit, so the miniaturized system is suitable for testing nanoparticles even in very small volumes.
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