izpis_h1_title_alt

Helicobacter colisuis sp. nov., isolated from caecal contents of domestic pigs (Sus scrofa domesticus)
ID Gruntar, Igor (Avtor), ID Kostanjšek, Rok (Avtor), ID Pirš, Tina (Avtor), ID Papić, Bojan (Avtor)

.pdfPDF - Predstavitvena datoteka, prenos (4,46 MB)
MD5: 157859754608572C63801249A247EA62
URLURL - Izvorni URL, za dostop obiščite https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005600 Povezava se odpre v novem oknu

Izvleček
Seven Helicobacter-like isolates were cultured from caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp. in 2015. The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates. Morphologically, the bacterial cells were spirilli-shaped and highly motile, 1–2 µm long and ≤0.5 µm wide, Gram-negative, oxidase-positive and catalase-positive. They could not be identified using the standard-prescribed biochemical tests and had uniform, unique and reproducible MALDI-TOF mass spectra that most closely matched those of Helicobacter pullorum. Three strains (11154-15$^T$, 14348–15 and 16470–15) underwent whole-genome sequencing. Analysis of 16S rRNA gene sequences revealed a high similarity (≥99.8 % identity) to Helicobacter canadensis. Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %). The core genome-based phylogeny confirmed that the new strains form a distinct clade most closely related to H. canadensis. The conducted polyphasic taxonomic analysis confirmed that the three strains represent a novel Helicobacter species for which the name Helicobacter colisuis sp. nov. is suggested, with strain 11154-15$^T$ (= DSM 113688$^T$ = CCUG 76053$^T$) as the type strain.

Jezik:Angleški jezik
Ključne besede:Helicobacter, polymerase chain reaction, Sus scrofa, caecum, domestic pig, Sus scrofa domesticus, novel species
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:VF - Veterinarska fakulteta
BF - Biotehniška fakulteta
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2022
Št. strani:7 str.
Številčenje:Vol. 72, iss. 11, art. 005600
PID:20.500.12556/RUL-142718 Povezava se odpre v novem oknu
UDK:636.09:579
ISSN pri članku:1466-5034
DOI:10.1099/ijsem.0.005600 Povezava se odpre v novem oknu
COBISS.SI-ID:130261763 Povezava se odpre v novem oknu
Datum objave v RUL:22.11.2022
Število ogledov:402
Število prenosov:197
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
:
Kopiraj citat
Objavi na:Bookmark and Share

Gradivo je del revije

Naslov:International journal of systematic and evolutionary microbiology
Skrajšan naslov:Int. j. syst. evol. microbiol.
Založnik:Microbiology Society
ISSN:1466-5034
COBISS.SI-ID:521767193 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Projekti

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P4-0092
Naslov:Zdravje živali, okolje in varna hrana

Podobna dela

Podobna dela v RUL:
Podobna dela v drugih slovenskih zbirkah:

Nazaj