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Application of FTIR spectroscopy to detect changes in skeletal muscle composition due to obesity with insulin resistance and STZ-induced diabetes
ID Zupančič, Barbara (Avtor), ID Umek, Nejc (Avtor), ID Ugwoke, Chiedozie Kenneth (Avtor), ID Cvetko, Erika (Avtor), ID Horvat, Simon (Avtor), ID Grdadolnik, Jože (Avtor)

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Izvleček
Age, obesity, and diabetes mellitus are pathophysiologically interconnected factors that significantly contribute to the global burden of non-communicable diseases. These metabolic conditions are associated with impaired insulin function, which disrupts the metabolism of carbohydrates, lipids, and proteins and can lead to structural and functional changes in skeletal muscle. Therefore, the alterations in the macromolecular composition of skeletal muscle may provide an indication of the underlying mechanisms of insulin-related disorders. The aim of this study was to investigate the potential of Fourier transform infrared (FTIR) spectroscopy to reveal the changes in macromolecular composition in weight-bearing and non-weight-bearing muscles of old, obese, insulin-resistant, and young streptozotocin (STZ)-induced diabetic mice. The efficiency of FTIR spectroscopy was evaluated by comparison with the results of gold-standard histochemical techniques. The differences in biomolecular phenotypes and the alterations in muscle composition in relation to their functional properties observed from FTIR spectra suggest that FTIR spectroscopy can detect most of the changes observed in muscle tissue by histochemical analyses and more. Therefore, it could be used as an effective alternative because it allows for the complete characterization of macromolecular composition in a single, relatively simple experiment, avoiding some obvious drawbacks of histochemical methods.

Jezik:Angleški jezik
Ključne besede:Fourier transform infrared (FTIR) spectroscopy, histochemical analysis, insulin resistance, obesity, diabetes, skeletal muscle macromolecular composition
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:MF - Medicinska fakulteta
BF - Biotehniška fakulteta
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2022
Št. strani:22 str.
Številčenje:Vol. 23, no. 20
PID:20.500.12556/RUL-142154 Povezava se odpre v novem oknu
UDK:616
ISSN pri članku:1422-0067
DOI:10.3390/ijms232012498 Povezava se odpre v novem oknu
COBISS.SI-ID:126210563 Povezava se odpre v novem oknu
Datum objave v RUL:21.10.2022
Število ogledov:815
Število prenosov:104
Metapodatki:XML DC-XML DC-RDF
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Gradivo je del revije

Naslov:International journal of molecular sciences
Skrajšan naslov:Int. j. mol. sci.
Založnik:MDPI
ISSN:1422-0067
COBISS.SI-ID:2779162 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:medicina, diabetes, debelost, mišji modeli, skeletne mišice, makromolekularna sestava, spektroskopija, FTIR

Projekti

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P1-0010
Naslov:Folding in dinamika biomolekularnih sistemov

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P3-0043
Naslov:Molekularni mehanizmi razvoja in delovanja skeletne mišice

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P4-0220
Naslov:Primerjalna genomika in genomska biodiverziteta

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