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Complex protein retention shifts with a pressure increase : an indication of a standard partial molar volume increase during adsorption?
ID
Kristl, Anja
(
Avtor
),
ID
Caf, Maja
(
Avtor
),
ID
Pompe, Matevž
(
Avtor
),
ID
Podgornik, Aleš
(
Avtor
)
PDF - Predstavitvena datoteka,
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(3,12 MB)
MD5: 7026125F9500085EB2331E1B628D9798
URL - Izvorni URL, za dostop obiščite
https://pubs.acs.org/doi/10.1021/acs.analchem.2c01809
Galerija slik
Izvleček
Studies of protein adsorption on reversed-phase and ion exchange stationary phases demonstrated an increase in retention with increasing pressure, which is interpreted as a standard partial molar volume decrease during the transition of the protein from a mobile to a stationary phase. Investigation of the pressure effect on the retention of lysozyme and IgG on a cation exchange column surprisingly revealed a negative retention trend with the increase of pressure. Further investigation of this phenomenon was performed with β-lactoglobulin, which enabled adsorption to be studied on both cation and anion exchange columns using the same mobile phase with a pH of 5.2. The same surface charge and standard partial molar volume in the mobile phase allowed us to examine only the effect of adsorption. Interestingly, a negative retention trend with a pressure increase occurred on an anion exchange column while a positive trend was present on a cation exchange column. This indicates that the interaction type governs the change in the standard partial molar volume during adsorption, which is independent of the applied pressure. Increasing the protein charge by decreasing the pH of the mobile phase to 4 reversed the retention trend (into a negative) with a pressure increase on the cation exchange column. A further decrease of the pH value resulted in an even more pronounced negative trend. This counterintuitive behavior indicates an increase in the standard partial molar volume during adsorption with the protein charge, possibly due to intermolecular repulsion of adsorbed protein molecules. While a detailed mechanism remains to be elucidated, presented results demonstrate the complexity of ion exchange interactions that can be investigated simply by changing the column pressure.
Jezik:
Angleški jezik
Ključne besede:
ion exchange
,
protein retention
,
high pressure
,
partial molar volume change
,
UHPLC
,
adsorption
,
peptides and proteins
,
phase transitions
,
phosphates
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
MF - Medicinska fakulteta
FKKT - Fakulteta za kemijo in kemijsko tehnologijo
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2022
Št. strani:
Str. 13350-13358
Številčenje:
Vol. 94, iss. 39
PID:
20.500.12556/RUL-141985
UDK:
543.544.5HPLC:66.081.312:577.112
ISSN pri članku:
0003-2700
DOI:
10.1021/acs.analchem.2c01809
COBISS.SI-ID:
125139971
Datum objave v RUL:
13.10.2022
Število ogledov:
603
Število prenosov:
115
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Objavi na:
Gradivo je del revije
Naslov:
Analytical chemistry
Skrajšan naslov:
Anal. chem.
Založnik:
American Chemical Society
ISSN:
0003-2700
COBISS.SI-ID:
5085703
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
ionska izmenjava
,
zadrževanje proteinov
,
visok tlak
,
sprememba parcialnega molskega volumna
,
UHPLC
Projekti
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P1-0153
Naslov:
Raziskave in razvoj analiznih metod in postopkov
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P1-0201
Naslov:
Fizikalna kemija
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
J2-9440
Naslov:
Karakterizacija fraktalnih struktur in povečevalni kriteriji njihove sinteze
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