Tumour budding and poorly differentiated clusters in colon cancer – different manifestations of partial epithelial–mesenchymal transition

Pavlič, Ana (avtor), Boštjančič, Emanuela (avtor), Kavalar, Rajko (avtor), Ilijevec, Bojan (avtor), Bonin, Serena (avtor), Zanconati, Fabrizio (avtor), Zidar, Nina (avtor)

Morphological features including infiltrative growth, tumour budding (TB), and poorly differentiated clusters (PDCs) have a firmly established negative predictive value in colorectal cancer (CRC). Despite extensive research, the mechanisms underlying different tumour growth patterns remain poorly understood. The aim of this study was to investigate the involvement of epithelial–mesenchymal transition (EMT) in TB and PDCs in CRC. Using laser-capture microdissection, we obtained distinct parts of the primary CRC including TB, PDCs, expansive tumour front, and the central part of the tumour, and analysed the expression of EMT-related markers, i.e. the miR-200 family, ZEB1/2, RND3, and CDH1. In TB, the miR-200 family and CDH1 were significantly downregulated, while ZEB2 was significantly upregulated. In PDCs, miR-141, miR-200c, and CDH1 were significantly downregulated. No significant differences were observed in the expression of any EMT-related markers between the expansive tumour front and the central part of the tumour. Our results suggest that both TB and PDCs are related to partial EMT. Discrete differences in morphology and expression of EMT-related markers between TB and PDCs indicate that they represent different manifestations of partial EMT. TB seems to be closer to complete EMT than PDCs.

• Jezik: Angleški jezik
• Ključne besede: colon cancer, colorectal cancer, epitheliall–mesenchymal transition, miRNA, partial epithelial–mesenchymal transition, poorly differentiated clusters, tumour budding
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2022
• Št. strani: Str. 278-288
• Številčenje: Vol. 258, iss. 3
• PID: 20.500.12556/RUL-141977
• UDK: 616
• ISSN pri članku: 0022-3417
• DOI: 10.1002/path.5998
• COBISS.SI-ID: 121432323
• Datum objave v RUL: 13.10.2022

Gradivo je del revije

• Naslov: The journal of pathology
• Skrajšan naslov: J. pathol.
• Založnik: Wiley, The Pathological Society of Great Britain and Ireland
• ISSN: 0022-3417
• COBISS.SI-ID: 4745738

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: rak debelega črevesa, kolorektalni rak, epitelno-mezenhimski prehod

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P3-0054
• Naslov: Patologija in molekularna genetika

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: J3-1754
• Naslov: Novi prognostični in diagnostični označevalci v razvoju raka debelega črevesa in danke