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Fast and simple LC-MS/MS method for rifampicin quantification in human plasma
ID
Temova Rakuša, Žane
(
Author
),
ID
Roškar, Robert
(
Author
),
ID
Klančar, Anita
(
Author
),
ID
Trdan Lušin, Tina
(
Author
),
ID
Faganeli, Nataša
(
Author
),
ID
Grabnar, Iztok
(
Author
),
ID
Mrhar, Aleš
(
Author
),
ID
Kristl, Albin
(
Author
),
ID
Trontelj, Jurij
(
Author
)
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https://www.hindawi.com/journals/ijac/2019/4848236/
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Abstract
A simple, fast, and cost-effective LC-MS/MS method for quantification of rifampicin in human plasma was developed and fully validated. The plasma samples containing rifampicin and isotopically labelled internal standard rifampicin D8, were cleaned up using a Captiva ND Lipids filtration plate. Chromatographic separation was achieved on an 1290 Infinity liquid chromatograph coupled to 6460 Triple Quadrupole operated in positive mode on a core-shell Kinetex C18 column (50 % 2.1 mm, 2.6 %m) by gradient elution using 0.1% formic acid in water and acetonitrile as a mobile phase. The proposed method is the fastest method published by now, both in terms of sample preparation (approximately one minute per sample) and chromatographic analysis (total run time 2.4 min). Another key benefit is the outstanding sensitivity and wide analytical range (5-40000 %g/L) with good linearity, accuracy, and precision. The method showed almost complete recovery (92%) and absence of any significant matrix effect as demonstrated by uniform responses from QC samples prepared in blood plasma from 6 volunteers (RSD <5%). The proposed method was successfully applied to rifampicin quantification in 340 patients' plasma samples, thus demonstrating its suitability for both therapeutic drug monitoring and pharmacokinetic analysis.
Language:
English
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
FFA - Faculty of Pharmacy
Publication status:
Published
Publication version:
Version of Record
Year:
2019
Number of pages:
Str. 1-7
Numbering:
art. 4848236, Vol. 2019
PID:
20.500.12556/RUL-141359
UDC:
543.544:616-002.5
ISSN on article:
1687-8779
DOI:
10.1155/2019/4848236
COBISS.SI-ID:
4670321
Publication date in RUL:
29.09.2022
Views:
517
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57
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Record is a part of a journal
Title:
International Journal of Analytical Chemistry (Online)
Shortened title:
Int. J. Anal. Chem. (Online)
Publisher:
Hindawi Publishing Corporation
ISSN:
1687-8779
COBISS.SI-ID:
522084377
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Secondary language
Language:
English
Keywords:
rifampicin
,
človeška plazma
,
kromatografska separacija
Projects
Funder:
ARRS - Slovenian Research Agency
Project number:
P1-0189-2018
Name:
Farmacevtska tehnologija: od dostavnih sistemov učinkovin do terapijskih izidov zdravil pri otrocih in starostnikih
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