Fast and simple LC-MS/MS method for rifampicin quantification in human plasma

Temova Rakuša, Žane (author), Roškar, Robert (author), Klančar, Anita (author), Trdan Lušin, Tina (author), Faganeli, Nataša (author), Grabnar, Iztok (author), Mrhar, Aleš (author), Kristl, Albin (author), Trontelj, Jurij (author)

A simple, fast, and cost-effective LC-MS/MS method for quantification of rifampicin in human plasma was developed and fully validated. The plasma samples containing rifampicin and isotopically labelled internal standard rifampicin D8, were cleaned up using a Captiva ND Lipids filtration plate. Chromatographic separation was achieved on an 1290 Infinity liquid chromatograph coupled to 6460 Triple Quadrupole operated in positive mode on a core-shell Kinetex C18 column (50 % 2.1 mm, 2.6 %m) by gradient elution using 0.1% formic acid in water and acetonitrile as a mobile phase. The proposed method is the fastest method published by now, both in terms of sample preparation (approximately one minute per sample) and chromatographic analysis (total run time 2.4 min). Another key benefit is the outstanding sensitivity and wide analytical range (5-40000 %g/L) with good linearity, accuracy, and precision. The method showed almost complete recovery (92%) and absence of any significant matrix effect as demonstrated by uniform responses from QC samples prepared in blood plasma from 6 volunteers (RSD <5%). The proposed method was successfully applied to rifampicin quantification in 340 patients' plasma samples, thus demonstrating its suitability for both therapeutic drug monitoring and pharmacokinetic analysis.

• Language: English
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FFA - Faculty of Pharmacy
• Publication status: Published
• Publication version: Version of Record
• Year: 2019
• Number of pages: Str. 1-7
• Numbering: art. 4848236, Vol. 2019
• PID: 20.500.12556/RUL-141359
• UDC: 543.544:616-002.5
• ISSN on article: 1687-8779
• DOI: 10.1155/2019/4848236
• COBISS.SI-ID: 4670321
• Publication date in RUL: 29.09.2022

Record is a part of a journal

• Title: International Journal of Analytical Chemistry (Online)
• Shortened title: Int. J. Anal. Chem. (Online)
• Publisher: Hindawi Publishing Corporation
• ISSN: 1687-8779
• COBISS.SI-ID: 522084377

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: English
• Keywords: rifampicin, človeška plazma, kromatografska separacija

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P1-0189-2018
• Name: Farmacevtska tehnologija: od dostavnih sistemov učinkovin do terapijskih izidov zdravil pri otrocih in starostnikih