20.500.12556/RUL-141359 Fast and simple LC-MS/MS method for rifampicin quantification in human plasma A simple, fast, and cost-effective LC-MS/MS method for quantification of rifampicin in human plasma was developed and fully validated. The plasma samples containing rifampicin and isotopically labelled internal standard rifampicin D8, were cleaned up using a Captiva ND Lipids filtration plate. Chromatographic separation was achieved on an 1290 Infinity liquid chromatograph coupled to 6460 Triple Quadrupole operated in positive mode on a core-shell Kinetex C18 column (50 % 2.1 mm, 2.6 %m) by gradient elution using 0.1% formic acid in water and acetonitrile as a mobile phase. The proposed method is the fastest method published by now, both in terms of sample preparation (approximately one minute per sample) and chromatographic analysis (total run time 2.4 min). Another key benefit is the outstanding sensitivity and wide analytical range (5-40000 %g/L) with good linearity, accuracy, and precision. The method showed almost complete recovery (92%) and absence of any significant matrix effect as demonstrated by uniform responses from QC samples prepared in blood plasma from 6 volunteers (RSD <5%). The proposed method was successfully applied to rifampicin quantification in 340 patients' plasma samples, thus demonstrating its suitability for both therapeutic drug monitoring and pharmacokinetic analysis. rifampicin človeška plazma kromatografska separacija true false true Angleški jezik Angleški jezik Članek v reviji 2022-09-29 07:36:16 2022-09-29 07:36:20 2022-09-30 04:10:24 0000-00-00 00:00:00 2019 0 0 Str. 1-7 Vol. 2019 art. 4848236 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543.544:616-002.5 1687-8779 10.1155/2019/4848236 4670321 4848236.pdf 4848236.pdf 1 3E3569EC33ADAFE49F89D7BFA5B25204 ede047ee38978f08db1395ce4d18a6627e862041d8bff13b57547a4ffcafc3da d1843644-3fb8-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=162399 https://www.hindawi.com/journals/ijac/2019/4848236/ 1 a419d653-3fb8-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=162398 Fakulteta za farmacijo 0 0 0