After a century of overuse of antibiotics, we are now dealing with a positive evolutionary feedback loop that has enabled the survival of more resistant microorganisms. Today, due to the resistance and non-specific action of antibiotics, we are returning to the discoveries of the 20th century. A good alternative to the use of antibiotics today is the alternative use of lytic bacteriophages, which in nature are natural killers of bacterial cells. Acne vulgaris is a chronic inflammatory disease in which the bacterium Cutibacterium acnes plays an important role in the development of the disease itself. In the future, infections caused by C. acnes could be treated with the help of bacteriophages, but as biological agents, they are much more demanding to store than chemical active agents. The aim of the thesis was to find and from the environment successfully isolate bacteriophages that infect C. acnes and to check their stability in serum formulations. Serum is a cosmetic product that is physically a viscous liquid with a smooth homogeneous structure, which is characterized by rapid absorption and the ability to penetrate deeper layers of the skin. Based on the obtained results of the stability study, we found that environmental factors and compositions of serums suitable for storage have a very important influence on the stability of the bacteriophage concentration. We also found that the concentrations of bacteriophages that infect C. acnes remain unchanged for a long time at lower storage temperatures with the appropriate composition of the serum and a sufficiently high initial concentration of the bacteriophage.
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