The derivatization of 6-aminocaproic acid was investigated by coupling the isoleucine ester with Cbz-protected 6-aminocaproic acid. The peptide was synthesized from the starting compounds using various coupling reagents - CDI, EDC and EEDQ. EDC proved to be the best reagent, yielding the purest product; the worst for this reaction, however, was EEDQ. To the resulting peptide, 4-pentynoic acid was additionally attached using the EDC reagent. The synthesis was successful as confirmed by the NMR spectra of the final compound. The final derivative of 6-aminocaproic acid is the N-terminus of the electrophilic probe, in which the remainder of the probe is a part of the thesis of other colleagues. The synthesis of this electrophilic probe does not fall within the scope of this task.
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