Experimental exposure to bisphenol A has minimal effects on bone tissue in growing rams : a preliminary study

Brankovič, Jana (author), Leskovec, Jakob (author), Šturm, Sabina (author), Cerkvenik, Vesna (author), Šterpin, Saša (author), Osredkar, Joško (author), Pogorevc, Estera (author), Antolinc, David (author), Vrecl, Milka (author)

Bisphenol A (BPA) is a well-known synthetic compound that belongs to the group of endocrine-disrupting chemicals. Although bone tissue is a target for these compounds, studies on BPA-related effects on bone morphology in farm animals are limited. In this preliminary study, we investigated the effects of short-term dietary BPA exposure on femoral morphology, metabolism, mineral content, and biomechanical behavior in rams aged 9–12 months. Fourteen rams of the Istrian Pramenka breed were randomly divided into a BPA group and a control group (seven rams/group) and exposed to 25 μg BPA/kg bw for 64 days in feed. Blood was collected for determination of bone turnover markers (procollagen N-terminal propeptide, C-terminal telopeptide), and femurs were assessed via computed tomography, histomorphometry, three-point bending test, and mineral analysis. BPA had no significant effects on most of the parameters studied. Only mineral analysis showed decreased manganese (50%; p ≤ 0.05) and increased copper content (25%; p ≤ 0.05) in the femurs of BPA-exposed rams. These results suggest that a 2-month, low-dose exposure to BPA in growing rams did not affect the histomorphology, metabolism, and biomechanical behavior of femurs; however, it affected the composition of microelements, which could affect the histometric and biophysical properties of bone in the long term.

• Language: English
• Keywords: bisphenol A, rams, serum bone biomarkers, bone geometry, trabecular bone, cortical bone, bone biomechanics, mineral content
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty; BF - Biotechnical Faculty; FFA - Faculty of Pharmacy; FGG - Faculty of Civil and Geodetic Engineering
• Publication status: Published
• Publication version: Version of Record
• Publication date: 25.08.2022
• Year: 2022
• Number of pages: 15 str.
• Numbering: Vol. 12, no. 17, art. 2179
• PID: 20.500.12556/RUL-138935
• UDC: 636.09:615.9
• ISSN on article: 2076-2615
• DOI: 10.3390/ani12172179
• COBISS.SI-ID: 119202307
• Publication date in RUL: 26.08.2022

Record is a part of a journal

• Title: Animals
• Shortened title: Animals
• Publisher: MDPI AG
• ISSN: 2076-2615
• COBISS.SI-ID: 519120409

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 25.08.2022