20.500.12556/RUL-138935 Experimental exposure to bisphenol A has minimal effects on bone tissue in growing rams a preliminary study Bisphenol A (BPA) is a well-known synthetic compound that belongs to the group of endocrine-disrupting chemicals. Although bone tissue is a target for these compounds, studies on BPA-related effects on bone morphology in farm animals are limited. In this preliminary study, we investigated the effects of short-term dietary BPA exposure on femoral morphology, metabolism, mineral content, and biomechanical behavior in rams aged 9–12 months. Fourteen rams of the Istrian Pramenka breed were randomly divided into a BPA group and a control group (seven rams/group) and exposed to 25 μg BPA/kg bw for 64 days in feed. Blood was collected for determination of bone turnover markers (procollagen N-terminal propeptide, C-terminal telopeptide), and femurs were assessed via computed tomography, histomorphometry, three-point bending test, and mineral analysis. BPA had no significant effects on most of the parameters studied. Only mineral analysis showed decreased manganese (50%; p ≤ 0.05) and increased copper content (25%; p ≤ 0.05) in the femurs of BPA-exposed rams. These results suggest that a 2-month, low-dose exposure to BPA in growing rams did not affect the histomorphology, metabolism, and biomechanical behavior of femurs; however, it affected the composition of microelements, which could affect the histometric and biophysical properties of bone in the long term. bisphenol A rams serum bone biomarkers bone geometry trabecular bone cortical bone bone biomechanics mineral content true false true Angleški jezik Angleški jezik Neznano 2022-08-26 07:38:11 2022-08-26 07:38:12 2022-11-02 11:56:17 0000-00-00 00:00:00 2022 0 0 15 str. no. 17, art. 2179 Vol. 12 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2022-08-25 636.09:615.9 2076-2615 10.3390/ani12172179 119202307 Experimental-Brankovic.pdf Experimental-Brankovic.pdf 1 B444E0F0B0BAEBFD9347C0A2F3E33729 5ac3cf086caf717bf828645949c715e1f920beecb3330f5e3cdde27039ea946b 4dc39c50-2503-11ed-92af-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=159440 https://www.mdpi.com/2076-2615/12/17/2179 Odprti dostop 1 4072926a-2501-11ed-92af-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=159436 Veterinarska fakulteta Biotehniška fakulteta Fakulteta za farmacijo Fakulteta za gradbeništvo in geodezijo 0 0 0