Human milk is the most suitable food for a newborn. Its composition is constantly changing and adapting to a newborn's needs, thus enabling optimal growth and development. The purpose of the master's thesis was to measure the composition of macronutrients in human milk and determine how individual macronutrients are affected by body mass index and age of the mother, sex of the newborn, single or twin birth, and mode of delivery. 41 volunteers who were hospitalized during July 2021 and March 2022 at the Gynecology Clinic, University Medical Center Ljubljana with newborns participated in the research. The content of proteins, fats, and carbohydrates was measured with the human milk analyzer Miris (Miris HMATM, Uppsala, Sweden). It was found that the milk composition of our volunteers was influenced only by the body mass index. Mature milk from overweight and obese mothers contained higher amounts of fat (p = 0.016). The age of the mother, the sex of the newborn, single or twin birth, and mode of delivery did not affect the macronutrient composition of breast milk. Results of research indicate that human milk macronutrient composition is adapting to child's needs regardless of differences among anthropometric characteristics.
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