izpis_h1_title_alt

Development of microsatellite markers for Tanacetum cinerariifolium (Trevis.) Sch. Bip., a plant with a large and highly repetitive genome
ID Varga, Filip (Avtor), ID Liber, Zlatko (Avtor), ID Jakše, Jernej (Avtor), ID Turudić, Ante (Avtor), ID Šatović, Zlatko (Avtor), ID Radosavljević, Ivan (Avtor), ID Jeran, Nina (Avtor), ID Grdiša, Martina (Avtor)

.pdfPDF - Predstavitvena datoteka, prenos (956,53 KB)
MD5: 1B371CF039C6D4B546EDF27C2A40BD7F
URLURL - Izvorni URL, za dostop obiščite https://www.mdpi.com/2223-7747/11/13/1778 Povezava se odpre v novem oknu

Izvleček
Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevis.) Sch. Bip.) is an outcrossing plant species (2n = 18) endemic to the eastern Adriatic coast and source of the natural insecticide pyrethrin. Due to the high repeatability and large genome (1C-value = 9.58 pg) our previous attempts to develop microsatellite markers using the traditional method were unsuccessful. Now we have used Illumina paired-end whole genome sequencing and developed a specific procedure to obtain useful microsatellite markers. A total of 796,130,142 high-quality reads (approx. 12.5× coverage) were assembled into 6,909,675 contigs using two approaches (de novo assembly and joining of overlapped pair-end reads). A total of 31,380 contigs contained one or more microsatellite sequences, of which di-(59.7%) and trinucleotide (25.9%) repeats were the most abundant. Contigs containing microsatellites were filtered according to various criteria to achieve better yield of functional markers. After two rounds of testing, 17 microsatellite markers were developed and characterized in one natural population. Twelve loci were selected for preliminary genetic diversity analysis of three natural populations. Neighbor-joining tree, based on the proportion of shared alleles distances, grouped individuals into clusters according to population affiliation. The availability of codominant SSR markers will allow analysis of genetic diversity and structure of natural Dalmatian pyrethrum populations as well as identification of breeding lines and cultivars.

Jezik:Angleški jezik
Ključne besede:Tanacetum cinerariifolium, Dalmatian pyrethrum, Asteraceae, genomic SSRs, microsatellite markers, SSR markers
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:BF - Biotehniška fakulteta
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2022
Št. strani:11 str.
Številčenje:Vol. 11, iss. 13, art. 1778
PID:20.500.12556/RUL-138300 Povezava se odpre v novem oknu
UDK:633
ISSN pri članku:2223-7747
DOI:10.3390/plants11131778 Povezava se odpre v novem oknu
COBISS.SI-ID:115302403 Povezava se odpre v novem oknu
Datum objave v RUL:14.07.2022
Število ogledov:1058
Število prenosov:123
Metapodatki:XML DC-XML DC-RDF
:
Kopiraj citat
Objavi na:Bookmark and Share

Gradivo je del revije

Naslov:Plants
Skrajšan naslov:Plants
Založnik:MDPI
ISSN:2223-7747
COBISS.SI-ID:523345433 Povezava se odpre v novem oknu

Licence

Licenca:CC BY-NC 4.0, Creative Commons Priznanje avtorstva-Nekomercialno 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by-nc/4.0/deed.sl
Opis:Licenca Creative Commons, ki prepoveduje komercialno uporabo, vendar uporabniki ne rabijo upravljati materialnih avtorskih pravic na izpeljanih delih z enako licenco.
Začetek licenciranja:14.07.2022

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:dalmatinski bolhač, genetika, mikrosatelitski markerji, genetska raznolikost, SSR markerji

Projekti

Financer:HRZZ - Croatian Science Foundation
Številka projekta:IP-06-2016-9034
Naslov:Genetic background of Dalmatian pyrethrum (Tanacetum cinerariifolium/Trevir./Sch. Bip.) insecticidal potential
Akronim:PyrDiv

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P4-0077
Naslov:Kmetijske rastline - genetika in sodobne tehnologije

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Centre of Excellence for Biodiversity and Molecular Plant Breeding (CoE CroP-BioDiv), Zagreb, Croatia
Številka projekta:KK.01.1.1.01.0005
Naslov:Biodiversity and Molecular Plant Breeding

Podobna dela

Podobna dela v RUL:
Podobna dela v drugih slovenskih zbirkah:

Nazaj