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Modulating role of co-solutes in complexation between bovine serum albumin and sodium polystyrene sulfonate
ID
Simončič, Matjaž
(
Avtor
),
ID
Lukšič, Miha
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(1,13 MB)
MD5: 1E79BBA6F979F47C7F093787FC6FD3C8
URL - Izvorni URL, za dostop obiščite
https://www.mdpi.com/2073-4360/14/6/1245
Galerija slik
Izvleček
The action of three types of co-solutes: (i) salts (NaCl, NaBr, NaI), (ii) polymer (polyethylene glycol; PEG-400, PEG-3000, PEG-20000), and (iii) sugars (sucrose, sucralose) on the complexation between bovine serum albumin (BSA) and sodium polystyrene sulfonate (NaPSS) was studied. Three critical pH parameters were extracted from the pH dependence of the solution’s turbidity: pH$_c$ corresponding to the formation of the soluble complexes, pH$_Φ$ corresponding to the formation of the insoluble complexes, and pH$_{opt}$ corresponding to the charge neutralization of the complexes. In the presence of salts, the formation of soluble and insoluble complexes as well as the charge neutralization of complexes was hindered, which is a consequence of the electrostatic screening of attractive interactions between BSA and NaPSS. Distinct anion-specific trends were observed in which the stabilizing effect of the salt increased in the order: NaCl < NaBr < NaI. The presence of PEG, regardless of its molecular weight, showed no measurable effect on the formation of soluble complexes. PEG-400 and PEG-3000 showed no effect on the formation of insoluble complexes, but PEG-20000 in high concentrations promoted their formation due to the molecular crowding effect. The presence of sugar molecules had little effect on BSA-NaPSS complexation. Sucralose showed a minor stabilizing effect with respect to the onset of complex formation, which was due to its propensity to the protein surface. This was confirmed by the fluorescence quenching assay (Stern-Volmer relationship) and all-atom MD simulations. This study highlights that when evaluating the modulatory effect of co-solutes on protein-polyelectrolyte interactions, (co-solute)-protein interactions and their subsequent impact on protein aggregation must also be considered.
Jezik:
Angleški jezik
Ključne besede:
protein-polyelectrolyte complexation
,
solid-liquid phase separation
,
sucrose
,
sucralose
,
molecular crowding
,
electrostatic sreening
,
protein-PE complexation
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
FKKT - Fakulteta za kemijo in kemijsko tehnologijo
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2022
Št. strani:
22 str.
Številčenje:
Vol. 14, iss. 6, art. 1245
PID:
20.500.12556/RUL-137517
UDK:
577.322
ISSN pri članku:
2073-4360
DOI:
10.3390/polym14061245
COBISS.SI-ID:
101645315
Datum objave v RUL:
20.06.2022
Število ogledov:
776
Število prenosov:
105
Metapodatki:
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Objavi na:
Gradivo je del revije
Naslov:
Polymers
Skrajšan naslov:
Polymers
Založnik:
Molecular Diversity Preservation International
ISSN:
2073-4360
COBISS.SI-ID:
517951257
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:
19.03.2022
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
kompleksacija protein-polielektrolit
,
fazna separacija trdno-tekoče
,
saharoza
,
sukraloza
,
molekulska gneča
,
elektrostatsko senčenje
Projekti
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P1-0201
Naslov:
Fizikalna kemija
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Program financ.:
Young researchers
Financer:
NIH - National Institutes of Health
Številka projekta:
RM1GM135136
Naslov:
Solvation modeling for next-gen biomolecule simulations
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
J1-1708
Naslov:
Raziskave agregacije proteinov v vodnih raztopinah soli in drugih topnih dodatkov
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
J1-2471
Naslov:
Kemijska karcinogeneza: mehanistični vpogled
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