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Extraction of bioactive metabolites from Achillea millefolium L. with choline chloride based natural deep eutectic solvents : a study of the antioxidant and antimicrobial activity
ID Ivanović, Milena (Avtor), ID Grujić, Dragana (Avtor), ID Cerar, Janez (Avtor), ID Islamčević Razboršek, Maša (Avtor), ID Topalić-Trivunović, Ljiljana (Avtor), ID Savić, Aleksandar (Avtor), ID Kočar, Drago (Avtor), ID Kolar, Mitja (Avtor)

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Izvleček
In this study, the extraction efficiency of natural deep eutectic solvents (NADES) based on choline chloride as a hydrogen bond acceptor (HBA) and five different hydrogen bond donors (HBD; lactic acid, 1,4-butanediol, 1,2-propanediol, fructose and urea) was evaluated for the first time for the isolation of valuable bioactive compounds from Achillea millefolium L. The phytochemical profiles of NADES extracts obtained after ultrasound-assisted extraction were evaluated both spectrophotometrically (total phenolic content (TPC) and antioxidant assays) and chromatographically (UHPLC-MS and HPLC-UV). The results were compared with those obtained with 80% ethanol, 80% methanol, and water. The highest TPC value was found in the lactic acid-based NADES (ChCl-LA), which correlated with the highest antioxidant activity determined by the FRAP analysis. On the other hand, the highest antiradical potential against ABTS$^{+•}$ was determined for urea-based NADES. Phenolic acids (chlorogenic acid and dicaffeoylquinic acid isomers), flavones (luteolin and apigenin), and their corresponding glucosides were determined as the dominant individual phenolic compounds in all extracts. The antibacterial and antifungal properties of the extracts obtained against four bacterial cultures and two yeasts were evaluated using two methods: the agar dilution method to obtain the minimum inhibitory concentration (MIC) and the minimum bactericidal or fungicidal concentration (MBC or MFC), and the disc diffusion method. ChCl-LA had the lowest MIC and MBC/MFC with respect to all microorganisms, with an MIC ranging from 0.05 mg mL$^{−1}$ to 0.8 mg mL$^{−1}$, while the water extract had the weakest inhibitory activity with MIC and MBC/MFC higher than 3.2 mg mL$^{−1}$.

Jezik:Angleški jezik
Ključne besede:natural deep eutectic solvents (NADES), density and viscosity, yarrow, phenolic compounds, antioxidant activity, antimicrobial activity
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FKKT - Fakulteta za kemijo in kemijsko tehnologijo
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2022
Št. strani:17 str.
Številčenje:Vol. 11, iss. 4, art. 724
PID:20.500.12556/RUL-137444 Povezava se odpre v novem oknu
UDK:543
ISSN pri članku:2076-3921
DOI:10.3390/antiox11040724 Povezava se odpre v novem oknu
COBISS.SI-ID:104614915 Povezava se odpre v novem oknu
Datum objave v RUL:17.06.2022
Število ogledov:400
Število prenosov:81
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
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Gradivo je del revije

Naslov:Antioxidants
Skrajšan naslov:Antioxidants
Založnik:MDPI
ISSN:2076-3921
COBISS.SI-ID:522976025 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:06.04.2022

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:viskoznost, fenoli, antioksidanti

Projekti

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P1-0153
Naslov:Raziskave in razvoj analiznih metod in postopkov

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P2-0006
Naslov:Fizikalno kemijski pojavi na površinskih plasteh in uporaba nanodelcev

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P1-0201
Naslov:Fizikalna kemija

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Republika of Srpska, Ministry of Scientific and Technological Development, Higher Education and Information Society
Številka projekta:19/06-020/964-21/18

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Republika of Srpska, Ministry of Scientific and Technological Development, Higher Education and Information Society
Številka projekta:19/06-020/961-64/18

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Slovenia, Ministry of Education, Science and Sport, Postdoctoral Contract
Številka projekta:OP20.04348

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