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Ibogaine-mediated ROS/antioxidant elevation in isolated rat uterus is β-adrenergic receptors and K$_{ATP}$ channels mediated
ID Tatalović, Nikola (Avtor), ID Vidonja-Uzelac, Teodora (Avtor), ID Oreščanin-Dušić, Zorana (Avtor), ID Nikolić-Kokić, Aleksandra (Avtor), ID Bresjanac, Mara (Avtor), ID Blagojević, Duško (Avtor)

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Izvleček
Ibogaine effects are mediated by cellular receptors, ATP depletion followed by ROS production and antioxidant enzyme activity elevation in a dose and time dependent manner. Since the role of K$_{ATP}$ channels and β-adrenoceptors in ROS cellular circuit was established here we explored their role in ibogaine pro-antioxidant effectiveness. Single dose of ibogaine (10 mg/L i.e., 28.8 µmol/L) was applied to isolated rat uterus (spontaneous and Ca$^{2+}$-stimulated) and contractility and antioxidant enzymes activity were monitored during 4 h. Ibogaine increased amplitude and frequency of spontaneous active uteri immediately after addition that was prevented by propranolol (β$_1$ and β$_2$ adrenoceptors selective antagonists) and glibenclamide (K$_{ATP}$ sensitive channels inhibitor; only frequency) pre-treatment. In Ca$^{2+}$-stimulated uteri, ibogaine decreased both amplitude and frequency after 4 h. Pre-treatment with propranolol abolished ibogaine induced amplitude lowering, while glibenclamide had no effect. In both types of active uterus, ibogaine induced a decrease in SOD1 and an increase in CAT activity after 2 h. In Ca$^{2+}$-stimulated uterus, there was also a decrease of SOD2 activity after 2 h. After 4 h, SOD1 activity returned to the baseline level, but GSH-Px activity increased. Pre-treatment with both propranolol and glibenclamide abolished observed changes of antioxidant enzymes activity suggesting that ibogaine pro-antioxidative effectiveness is β-adrenergic receptors and K$_{ATP}$ channels mediated.

Jezik:Angleški jezik
Ključne besede:ibogaine, K$_{ATP}$ channels, glibenclamide, β-adrenergic receptors, propranolol, antioxidative enzymes, superoxide dismutase, catalase, uterus, contractility
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:MF - Medicinska fakulteta
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2021
Št. strani:16 str.
Številčenje:Vol. 10, iss. 11, art. 1792
PID:20.500.12556/RUL-137417 Povezava se odpre v novem oknu
ISSN pri članku:2076-3921
DOI:10.3390/antiox10111792 Povezava se odpre v novem oknu
COBISS.SI-ID:140497923 Povezava se odpre v novem oknu
Datum objave v RUL:16.06.2022
Število ogledov:632
Število prenosov:104
Metapodatki:XML DC-XML DC-RDF
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Gradivo je del revije

Naslov:Antioxidants
Skrajšan naslov:Antioxidants
Založnik:MDPI
ISSN:2076-3921
COBISS.SI-ID:522976025 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:ibogain, K$_{ATP}$ kanali, glibenklamid, β-adrenergični receptorji, propranolol, antioksidativni encimi, superoksid dismutaza, katalaza, maternica, kontraktilnost

Projekti

Financer:MESTD - Ministry of Education, Science and Technological Development of Republic of Serbia
Številka projekta:451-03-68/2020-14/200007

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P3-0171
Naslov:Plastičnost živčevja v fizioloških in patofizioloških razmerah

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