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Toxicity of the diatom genus Pseudo-nitzschia (Bacillariophyceae) : insights from toxicity tests and genetic screening in the Northern Adriatic Sea
ID
Turk Dermastia, Timotej
(
Avtor
),
ID
Dall’Ara, Sonia
(
Avtor
),
ID
Dolenc, Jožica
(
Avtor
),
ID
Mozetič, Patricija
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(2,34 MB)
MD5: 53B2E676ED684509C044AC7D5C76C24B
URL - Izvorni URL, za dostop obiščite
https://www.mdpi.com/2072-6651/14/1/60
Galerija slik
Izvleček
Diatoms of the genus Pseudo-nitzschia H. Peragallo are known to produce domoic acid (DA), a toxin involved in amnesic shellfish poisoning (ASP). Strains of the same species are often classified as both toxic and nontoxic, and it is largely unknown whether this difference is also genetic. In the Northern Adriatic Sea, there are virtually no cases of ASP, but DA occasionally occurs in shellfish samples. So far, three species—P. delicatissima (Cleve) Heiden, P. multistriata (H. Takano) H. Takano, and P. calliantha Lundholm, Moestrup, & Hasle—have been identified as producers of DA in the Adriatic Sea. By means of enzme-linked immunosorbent assay (ELISA), high-performance liquid chromatography with UV and visible spectrum detection (HPLC-UV/VIS), and liquid chromatography with tandem mass spectrometry (LC-MS/MS), we reconfirmed the presence of DA in P. multistriata and P. delicatissima and detect for the first time in the Adriatic Sea DA in P. galaxiae Lundholm, & Moestrup. Furthermore, we attempted to answer the question of the distribution of DA production among Pseudo-nitzschia species and strains by sequencing the internal transcribed spacer (ITS) phylogenetic marker and the dabA DA biosynthesis gene and coupling this with toxicity data. Results show that all subclades of the Pseudo-nitzschia genus contain toxic species and that toxicity appears to be strain dependent, often with geographic partitioning. Amplification of dabA was successful only in toxic strains of P. multistriata and the presence of the genetic architecture for DA production in non-toxic strains was thus not confirmed.
Jezik:
Angleški jezik
Ključne besede:
Adriatic
,
dabA
,
domoic acid
,
Pseudo-nitzschia galaxiae
,
ITS
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
VF - Veterinarska fakulteta
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2022
Št. strani:
17 str.
Številčenje:
Vol. 14, iss. 1, art. 60
PID:
20.500.12556/RUL-137126
UDK:
615.9
ISSN pri članku:
2072-6651
DOI:
10.3390/toxins14010060
COBISS.SI-ID:
94153219
Datum objave v RUL:
01.06.2022
Število ogledov:
672
Število prenosov:
113
Metapodatki:
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Objavi na:
Gradivo je del revije
Naslov:
Toxins. Elektronski vir
Skrajšan naslov:
Toxins
Založnik:
MDPI
ISSN:
2072-6651
COBISS.SI-ID:
517594649
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:
15.01.2022
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
toksikologija
,
diatomeje
Projekti
Financer:
EC - European Commission
Program financ.:
European Regional Development Fund, Operational Programme for the Implementation of the EU Cohesion Policy
Naslov:
Development of research infrastructure for international competitiveness of Slovene development of research infrastructure area
Akronim:
RI-SI-2 LifeWatch
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
Republic of Slovenia, Ministry of Education, Science and Sport
Naslov:
Development of research infrastructure for international competitiveness of Slovene development of research infrastructure area
Akronim:
RI-SI-2 LifeWatch
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P1-0237
Naslov:
Raziskave obalnega morja
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Program financ.:
Young researchers
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