izpis_h1_title_alt

Elimination of eight viruses and two viroids from preclonal candidates of six grapevine varieties (Vitis vinifera L.) through in vivo thermotherapy and in vitro meristem tip micrografting
ID Miljanić, Vanja (Avtor), ID Rusjan, Denis (Avtor), ID Škvarč, Andreja (Avtor), ID Chatelet, Philippe (Avtor), ID Štajner, Nataša (Avtor)

.pdfPDF - Predstavitvena datoteka, prenos (3,73 MB)
MD5: F6EF086BC87D2149F8CDDA0225197737
URLURL - Izvorni URL, za dostop obiščite https://www.mdpi.com/2223-7747/11/8/1064 Povezava se odpre v novem oknu

Izvleček
Viruses and virus-like organisms are amajor problemin viticultureworldwide. They cannot be controlled by standard plant protection measures, and once infected, plants remain infected throughout their life; therefore, the propagation of healthy vegetative material is crucial. In vivo thermotherapy at 36–38 °C for at least six weeks, followed by meristem tip micrografting (0.1–0.2 mm) onto in vitro-growing seedling rootstocks of Vialla (Vitis labrusca × Vitis riparia), was successfully used to eliminate eight viruses (grapevine rupestris stem pitting-associated virus (GRSPaV), grapevine Pinot gris virus (GPGV), grapevine fanleaf virus (GFLV), grapevine leafroll-associated virus 3 (GLRaV-3), grapevine fleck virus (GFkV), grapevine rupestris vein feathering virus (GRVFV), grapevine Syrah virus-1 (GSyV-1), and raspberry bushy dwarf virus (RBDV)), as well as two viroids (hop stunt viroid (HSVd) and grapevine yellow speckle viroid 1 (GYSVd-1)) from preclonal candidates of six grapevine varieties (Vitis vinifera L.). A half-strength MS medium including vitamins supplemented with 30 g/L of sucrose and solidified with 8 g/L of agar, without plant growth regulators, was used for the growth and root development of micrografts and the subsequently micropropagated plants; no callus formation, hyperhydricity, or necrosis of shoot tips was observed. Although the overall regeneration was low (higher in white than in red varieties), a 100% elimination was achieved for all eight viruses, whereas the elimination level for viroids was lower, reaching only 39.2% of HSVd-free and 42.6% GYSVd-1-free vines. To the best of our knowledge, this is the first report of GPGV, GRVFV, GSyV-1, HSVd, and GYSVd-1 elimination through combining in vivo thermotherapy and in vitro meristem tip micrografting, and the first report of RBDV elimination from grapevines. The virus-free vines were successfully acclimatized in rockwool plugs and then transferred to soil.

Jezik:Angleški jezik
Ključne besede:Vitis vinifera L., grapevine, viruses, viroids, micrografting, thermotherapy
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:BF - Biotehniška fakulteta
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2022
Št. strani:14 str.
Številčenje:Vol. 11, iss. 8, art. 1064
PID:20.500.12556/RUL-136431 Povezava se odpre v novem oknu
UDK:634.8
ISSN pri članku:2223-7747
DOI:10.3390/plants11081064 Povezava se odpre v novem oknu
COBISS.SI-ID:105430787 Povezava se odpre v novem oknu
Datum objave v RUL:04.05.2022
Število ogledov:770
Število prenosov:136
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
:
Kopiraj citat
Objavi na:Bookmark and Share

Gradivo je del revije

Naslov:Plants
Skrajšan naslov:Plants
Založnik:MDPI
ISSN:2223-7747
COBISS.SI-ID:523345433 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:vinska trta, virusi, viroidi, termoterapija, cepljenje, tkivne kulture

Projekti

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P4-0077
Naslov:Kmetijske rastline - genetika in sodobne tehnologije

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Public Scholarship, Development, Disability, and Maintenance Fund of the Republic of Slovenia, Scholarship for nationals of the Western Balkans to postgraduate study in Slovenia

Podobna dela

Podobna dela v RUL:
Podobna dela v drugih slovenskih zbirkah:

Nazaj