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Phenolic profile, antioxidant capacity and antimicrobial activity of nettle leaves extracts obtained by advanced extraction techniques
ID
Elez-Garofulić, Ivona
(
Avtor
),
ID
Malin, Valentina
(
Avtor
),
ID
Repajić, Maja
(
Avtor
),
ID
Zorić, Zoran
(
Avtor
),
ID
Pedisić, Sandra
(
Avtor
),
ID
Sterniša, Meta
(
Avtor
),
ID
Smole Možina, Sonja
(
Avtor
),
ID
Dragović-Uzelac, Verica
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(966,32 KB)
MD5: F946C7DA4DCB444A17684BE82ECE7D00
URL - Izvorni URL, za dostop obiščite
https://www.mdpi.com/1420-3049/26/20/6153
Galerija slik
Izvleček
Nettle is a widely known plant whose high biological activity and beneficial medicinal effects are attributed to various bioactive compounds, among which polyphenols play an important role. In order to isolate polyphenols and preserve their properties, advanced extraction techniques have been applied to overcome the drawbacks of conventional ones. Therefore, microwave-assisted extraction (MAE) has been optimized for the isolation of nettle leaves polyphenols and it was compared to pressurized liquid extraction (PLE) and conventional heat-reflux extraction (CE). The obtained extracts were analyzed for their individual phenolic profile by UPLC MS$^2$ and for their antioxidant capacity by ORAC assay. MAE proved to be the more specific technique for the isolation of individual phenolic compounds, while PLE produced extracts with higher amount of total phenols and higher antioxidant capacity. Both techniques were more effective compared to CE. PLE nettle extract showed antimicrobial activity against bacteria, especially against Gram-negative Pseudomonas fragi ATCC 4973 and Campylobacter jejuni NCTC 11168 strains. This suggests that PLE is suitable for obtaining a nettle extract with antioxidant and antimicrobial potential, which as such has great potential for use as a value-added ingredient in the food and pharmaceutical industry.
Jezik:
Angleški jezik
Ključne besede:
nettle leaves
,
microwave-assisted extraction
,
pressurized liquid extraction
,
advanced extraction techniques
,
plant polyphenols
,
antioxidant capacity
,
ORAC
,
UPLC MS$^2$
,
antimicrobial activity
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
BF - Biotehniška fakulteta
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2021
Št. strani:
16 str.
Številčenje:
Vol. 26, iss. 20, art. 6153
PID:
20.500.12556/RUL-136263
UDK:
547.9:582.635.8:66.061
ISSN pri članku:
1420-3049
DOI:
10.3390/molecules26206153
COBISS.SI-ID:
80267267
Datum objave v RUL:
21.04.2022
Število ogledov:
1015
Število prenosov:
104
Metapodatki:
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Objavi na:
Gradivo je del revije
Naslov:
Molecules
Skrajšan naslov:
Molecules
Založnik:
MDPI
ISSN:
1420-3049
COBISS.SI-ID:
18462981
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:
12.10.2021
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
rastlinski izvlečki
,
kopriva
,
ekstrakcija
,
ekstrakcijske metode
,
fenolne spojine
,
antioksidativni potencial
,
protimikrobno delovanje
Projekti
Financer:
HRZZ - Croatian Science Foundation
Številka projekta:
IP-01-2018-4924
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P4-0116
Naslov:
Mikrobiologija in biotehnologija živil in okolja
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
CEEPUS
Številka projekta:
CIII-HR-0306-12-1920- M-140167
Naslov:
For Safe and Healthy Food in Middle Europe
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