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The effect of the osmotically active compound concentration difference on the passive water and proton fluxes across a lipid bilayer
ID
Przybyło, Magdalena
(
Avtor
),
ID
Drabik, Dominik
(
Avtor
),
ID
Doskocz, Joanna
(
Avtor
),
ID
Iglič, Aleš
(
Avtor
),
ID
Langner, Marek
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(2,79 MB)
MD5: 58C9438376F56AC2EA1D7AF87D0C1A0E
URL - Izvorni URL, za dostop obiščite
https://www.mdpi.com/1422-0067/22/20/11099
Galerija slik
Izvleček
The molecular details of the passive water flux across the hydrophobic membrane interior are still a matter of debate. One of the postulated mechanisms is the spontaneous, water-filled pore opening, which facilitates the hydrophilic connection between aqueous phases separated by the membrane. In the paper, we provide experimental evidence showing that the spontaneous lipid pore formation correlates with the membrane mechanics; hence, it depends on the composition of the lipid bilayer and the concentration of the osmotically active compound. Using liposomes as an experimental membrane model, osmotically induced water efflux was measured with the stopped-flow technique. Shapes of kinetic curves obtained at low osmotic pressure differences are interpreted in terms of two events: the lipid pore opening and water flow across the aqueous channel. The biological significance of the dependence of the lipid pore formation on the concentration difference of an osmotically active compound was illustrated by the demonstration that osmotically driven water flow can be accompanied by the dissipation of the pH gradient. The application of the Helfrich model to describe the probability of lipid pore opening was validated by demonstrating that the probability of pore opening correlates with the membrane bending rigidity. The correlation was determined by experimentally derived bending rigidity coefficients and probabilities of lipid pores opening.
Jezik:
Angleški jezik
Ključne besede:
lipid bilayer
,
water transport
,
proton transport
,
water activity
,
membrane mechanics
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
FE - Fakulteta za elektrotehniko
MF - Medicinska fakulteta
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2021
Št. strani:
19 str.
Številčenje:
Vol. 22, iss. 20, art. 11099
PID:
20.500.12556/RUL-136231
UDK:
577
ISSN pri članku:
1422-0067
DOI:
10.3390/ijms222011099
COBISS.SI-ID:
81110275
Datum objave v RUL:
20.04.2022
Število ogledov:
630
Število prenosov:
97
Metapodatki:
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Objavi na:
Gradivo je del revije
Naslov:
International journal of molecular sciences
Skrajšan naslov:
Int. j. mol. sci.
Založnik:
MDPI
ISSN:
1422-0067
COBISS.SI-ID:
2779162
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:
14.10.2021
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
lipidni dvosloj
,
transport vode
,
transport protona
,
vodna aktivnost
,
membranska mehanika
Projekti
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
Department of Biomedical Engineering
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
Lipid Systems Co. Ltd.
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P2-0232
Naslov:
Funkcije in tehnologije kompleksnih sistemov
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
National Centre for Research and Development
Številka projekta:
POIR.04.01.04-00-0050/15
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