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Adhesion and stiffness of detached breast cancer cells in vitro : co-treatment with metformin and 2-deoxy-D-glucose induces changes related to increased metastatic potential
ID
Zemljič Jokhadar, Špela
(
Avtor
),
ID
Kokot, Gašper
(
Avtor
),
ID
Pavlin, Mojca
(
Avtor
),
ID
Derganc, Jure
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(2,29 MB)
MD5: 94095E2946C87D535C1B1E4C21754D43
URL - Izvorni URL, za dostop obiščite
https://www.mdpi.com/2079-7737/10/9/873
Galerija slik
Izvleček
Metastatic cancer cells can overcome detachment-induced cell death and can proliferate in anchorage-independent conditions. A recent study revealed that a co-treatment with two drugs that interfere with cell metabolism, metformin and 2-deoxy-D-glucose, promotes detachment of viable MDA-MB-231 breast cancer cells. In the present study, we analyzed if these detached viable MDA-MB-231 cells also exhibit other features related to cancer metastatic potential, i.e., if they are softer and more prone to adhere to epithelial cells. The cell mechanics of attached cells and floating cells were analyzed by optical tweezers and cell deformability cytometry, respectively. The adhesion was assessed on a confluent monolayer of HUVEC cells, with MDA-MB-231 cells either in static conditions or in a microfluidic flow. Additionally, to test if adhesion was affected by the state of the epithelial glycocalyx, HUVEC cells were treated with neuraminidase and tunicamycin. It was found that the treated MDA-MB-231 cells were more prone to adhere to HUVEC cells and that they were softer than the control, both in the floating state and after re-seeding to a substrate. The changes in the HUVEC glycocalyx, however, did not increase the adhesion potential of MDA-MB-231.
Jezik:
Angleški jezik
Ključne besede:
metastatic potential
,
adhesion to epithelial cells
,
optical tweezers
,
cell deformability cytometry
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
MF - Medicinska fakulteta
FE - Fakulteta za elektrotehniko
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2021
Št. strani:
15 str.
Številčenje:
Vol. 10, iss. 9, art. 873
PID:
20.500.12556/RUL-136166
UDK:
616-006
ISSN pri članku:
2079-7737
DOI:
10.3390/biology10090873
COBISS.SI-ID:
87303939
Datum objave v RUL:
14.04.2022
Število ogledov:
634
Število prenosov:
97
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Objavi na:
Gradivo je del revije
Naslov:
Biology
Skrajšan naslov:
Biology
Založnik:
MDPI
ISSN:
2079-7737
COBISS.SI-ID:
523004441
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:
04.09.2021
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
metastatski potencial
,
adhezija na epitelijske celice
,
optične pincete
Projekti
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P1-0055
Naslov:
Biofizika polimerov, membran, gelov, koloidov in celic
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
J3-6794
Naslov:
Celična energijska presnova kot tarča za zdravljenje raka – genski in farmakološki pristop
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