20.500.12556/RUL-136166 Adhesion and stiffness of detached breast cancer cells in vitro co-treatment with metformin and 2-deoxy-D-glucose induces changes related to increased metastatic potential Metastatic cancer cells can overcome detachment-induced cell death and can proliferate in anchorage-independent conditions. A recent study revealed that a co-treatment with two drugs that interfere with cell metabolism, metformin and 2-deoxy-D-glucose, promotes detachment of viable MDA-MB-231 breast cancer cells. In the present study, we analyzed if these detached viable MDA-MB-231 cells also exhibit other features related to cancer metastatic potential, i.e., if they are softer and more prone to adhere to epithelial cells. The cell mechanics of attached cells and floating cells were analyzed by optical tweezers and cell deformability cytometry, respectively. The adhesion was assessed on a confluent monolayer of HUVEC cells, with MDA-MB-231 cells either in static conditions or in a microfluidic flow. Additionally, to test if adhesion was affected by the state of the epithelial glycocalyx, HUVEC cells were treated with neuraminidase and tunicamycin. It was found that the treated MDA-MB-231 cells were more prone to adhere to HUVEC cells and that they were softer than the control, both in the floating state and after re-seeding to a substrate. The changes in the HUVEC glycocalyx, however, did not increase the adhesion potential of MDA-MB-231. metastatic potential adhesion to epithelial cells optical tweezers cell deformability cytometry metastatski potencial adhezija na epitelijske celice optične pincete true false true Angleški jezik Slovenski jezik Članek v reviji 2022-04-14 09:04:58 2022-04-14 09:05:00 2022-09-15 04:13:29 0000-00-00 00:00:00 2021 0 0 15 str. iss. 9, art. 873 Vol. 10 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-006 2079-7737 10.3390/biology10090873 87303939 biology-10-00873-v3.pdf biology-10-00873-v3.pdf 1 94095E2946C87D535C1B1E4C21754D43 056b3459922023254d62348faefa1738dd09f50da8cbadad0ccd1e58dfb4f307 56db8027-bbc1-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155661 https://www.mdpi.com/2079-7737/10/9/873 1 250640e2-bbc1-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155660 Medicinska fakulteta Fakulteta za elektrotehniko 0 0 0