Research has classified industrial hemp (Cannabis sativa L.) as a fast-growing, undemanding, hyperaccumulative plant for decades. It is also attributed a high level of heavy metal uptake, while genes responsive to the presence of potentially toxic metals in industrial hemp have not been sequenced and annotated, and their involvement in their uptake is not well understood. The study tries to fill the gap of conflicting or incomplete data in literature, and investigates the involvemenet of GSR 1, PLD α, HSP70, PDR 12, and HMA 4 genes in the response of industrial hemp to potentially toxic metals. The study is based on our own experimental data and analyses in a controlled pot experiment, whereby we evaluated the involvement of genes in the response to two potentially toxic metals, lead and cadmium, in two separate varieties of hemp, Futura 75 and Tisza. By designing the initial oligonucleotides on the basis of interspecific preserved gene sequences - orthologs in already annotated species such as Arabidopsis thalinana, Brassica juncea, Lactuca sativa, Triticum urartu and Vitis vinifera, the study also offers an indirect insight into the similarity of nucleotide sequences of these genes in industrial hemp.
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