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Stability-indicating analytical approach for stability evaluation of lactoferrin
ID Osel, Nika (Avtor), ID Planinšek Parfant, Timeja (Avtor), ID Kristl, Albin (Avtor), ID Roškar, Robert (Avtor)

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Izvleček
Lactoferrin is a multifunctional iron-binding glycoprotein in milk. Due to its potential for the treatment of various diseases, interest in products containing lactoferrin is increasing. However, as a protein, it is prone to degradation, which critically affects the quality of products. Therefore, the main purpose of our work was to develop a stability-indicating analytical approach for stability evaluation of lactoferrin. We were focused on two complementary methods: reversed-phase and size-exclusion chromatography. The stability-indicating nature of the selected methods was confirmed. They were successfully validated by following the ICH guidelines and applied to preliminary lactoferrin stability studies. Up to three degradation products, as well as aggregates and fragments of lactoferrin, were detected in various samples using complementary reversed-phase and size-exclusion chromatographic methods. The analytical approach was additionally extended with three spectroscopic techniques (absorbance, intrinsic fluorescence, and bicinchoninic acid method), which may provide valuable complementary information in some cases. The presented analytical approach allows the stability evaluation of lactoferrin in various samples, including the ability to detect differences in its degradation mechanisms. Furthermore, it has the potential to be used for the quality control of products containing lactoferrin.

Jezik:Angleški jezik
Ključne besede:lactoferrin, stability, HPLC-UV, spectroscopic methods, stability-indicating approach, proteins, quality control
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FFA - Fakulteta za farmacijo
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2021
Št. strani:24 str.
Številčenje:Vol. 13, iss. 7, art. 1065
PID:20.500.12556/RUL-135599 Povezava se odpre v novem oknu
UDK:66.022.362:535.33/.34:615
ISSN pri članku:1999-4923
DOI:10.3390/pharmaceutics13071065 Povezava se odpre v novem oknu
COBISS.SI-ID:70061059 Povezava se odpre v novem oknu
Datum objave v RUL:22.03.2022
Število ogledov:5610
Število prenosov:98
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Gradivo je del revije

Naslov:Pharmaceutics
Skrajšan naslov:Pharmaceutics
Založnik:MDPI
ISSN:1999-4923
COBISS.SI-ID:517949977 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:proteini, stabilnost, laktoferin, nadzor kakovosti, spektroskopska analiza

Projekti

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P1-0189
Naslov:Farmacevtska tehnologija: od dostavnih sistemov učinkovin do terapijskih izidov zdravil pri otrocih in starostnikih

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Support of research and development projects (TRL 3–6), S4—Networks for the transition to a circular economy, Biomass and alternative raw materials
Naslov:Fractionation and processing of whey proteins and exploitation of the residue for the formation of new functional foods and food supplements
Akronim:LAKTIKA

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