Differential response of grapevine to infection with ʼCandidatus Phytoplasma solaniʼ in early and late growing season through complex regulation of mRNA and small RNA transcriptomes
ID Dermastia, Marina (Author), ID Škrlj, Blaž (Author), ID Strah, Rebeka (Author), ID Anžič, Barbara (Author), ID Tomaž, Špela (Author), ID Križnik, Maja (Author), ID Schönhuber, Christina (Author), ID Riedle-Bauer, Monika (Author), ID Ramšak, Živa (Author), ID Petek, Marko (Author), ID Kladnik, Aleš (Author), ID Lavrač, Nada (Author), ID Gruden, Kristina (Author), ID Roitsch, Thomas (Author), ID Brader, Günter (Author), ID Pompe Novak, Maruša (Author)

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Bois noir is the most widespread phytoplasma grapevine disease in Europe. It is associated with ‘Candidatus Phytoplasma solani’, but molecular interactions between the causal pathogen and its host plant are not well understood. In this work, we combined the analysis of high-throughput RNA-Seq and sRNA-Seq data with interaction network analysis for finding new cross-talks among pathways involved in infection of grapevine cv. Zweigelt with ‘Ca. P. solani’ in early and late growing seasons. While the early growing season was very dynamic at the transcriptional level in asymptomatic grapevines, the regulation at the level of small RNAs was more pronounced later in the season when symptoms developed in infected grapevines. Most differentially expressed small RNAs were associated with biotic stress. Our study also exposes the less-studied role of hormones in disease development and shows that hormonal balance was already perturbed before symptoms development in infected grapevines. Analysis at the level of communities of genes and mRNAmicroRNA interaction networks revealed several new genes (e.g., expansins and cryptdin) that have not been associated with phytoplasma pathogenicity previously. These novel actors may present a new reference framework for research and diagnostics of phytoplasma diseases of grapevine.

Keywords:‘Candidatus Phytoplasma solani’, grapevine, bois noir, RNA-Seq, sRNA-Seq, miRNA, phasiRNA, hormones, interaction network
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Publication status:Published
Publication version:Version of Record
Number of pages:28 str.
Numbering:Vol. 22, iss. 7, art. 3531
PID:20.500.12556/RUL-135200 This link opens in a new window
ISSN on article:1661-6596
DOI:10.3390/ijms22073531 This link opens in a new window
COBISS.SI-ID:58895619 This link opens in a new window
Publication date in RUL:28.02.2022
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Record is a part of a journal

Title:International journal of molecular sciences
Shortened title:Int. j. mol. sci.
Publisher:Molecular Diversity Preservation International MDPI
COBISS.SI-ID:36217605 This link opens in a new window


License:CC BY 4.0, Creative Commons Attribution 4.0 International
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:01.04.2021

Secondary language

Keywords:ʼCandidatus Phytoplasma solaniʼ, vinska trta, počrnelost lesa, RNA-Seq, sRNA-Seq, miRNA, phasiRNA, hormoni, interakcijska omrežja


Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:J4-2544
Name:Ciljana mutageneza s CRISPR/CAS9 za odpornost vinske trte in krompirja proti fitoplazmam

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:N2-0078
Name:Semantično rudarjenje prostodostopnih povezanih podatkov

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:P4-0165
Name:Biotehnologija in sistemska biologija rastlin

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:P2-0103
Name:Tehnologije znanja

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Funding programme:Young Researcher

Funder:FWF - Austrian Science Fund
Project number:I 5042-B

Funder:FWF - Austrian Science Fund
Project number:I 2763-B29

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