20.500.12556/RUL-135200 Differential response of grapevine to infection with ʼCandidatus Phytoplasma solaniʼ in early and late growing season through complex regulation of mRNA and small RNA transcriptomes Bois noir is the most widespread phytoplasma grapevine disease in Europe. It is associated with ‘Candidatus Phytoplasma solani’, but molecular interactions between the causal pathogen and its host plant are not well understood. In this work, we combined the analysis of high-throughput RNA-Seq and sRNA-Seq data with interaction network analysis for finding new cross-talks among pathways involved in infection of grapevine cv. Zweigelt with ‘Ca. P. solani’ in early and late growing seasons. While the early growing season was very dynamic at the transcriptional level in asymptomatic grapevines, the regulation at the level of small RNAs was more pronounced later in the season when symptoms developed in infected grapevines. Most differentially expressed small RNAs were associated with biotic stress. Our study also exposes the less-studied role of hormones in disease development and shows that hormonal balance was already perturbed before symptoms development in infected grapevines. Analysis at the level of communities of genes and mRNAmicroRNA interaction networks revealed several new genes (e.g., expansins and cryptdin) that have not been associated with phytoplasma pathogenicity previously. These novel actors may present a new reference framework for research and diagnostics of phytoplasma diseases of grapevine. ‘Candidatus Phytoplasma solani’ grapevine bois noir RNA-Seq sRNA-Seq miRNA phasiRNA hormones interaction network ʼCandidatus Phytoplasma solaniʼ vinska trta počrnelost lesa RNA-Seq sRNA-Seq miRNA phasiRNA hormoni interakcijska omrežja true false true Angleški jezik Slovenski jezik Članek v reviji 2022-02-28 14:13:01 2022-02-28 14:13:02 2022-09-14 04:12:08 0000-00-00 00:00:00 2021 0 0 28 str. iss. 7, art. 3531 Vol. 22 Apr. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 1661-6596 10.3390/ijms22073531 58895619 RAZ_Dermastia_Marina_2021.pdf RAZ_Dermastia_Marina_2021.pdf 1 2E141F52124D0E9D062B5054ECA4E4E8 5c1f060c153532d68b04eb508c2b3143608b09fa2c06234e1c8003d7bde786b6 3a7c1e91-9898-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154109 https://www.mdpi.com/1422-0067/22/7/3531 1 e5725844-9897-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154108 Biotehniška fakulteta 0 0 0