Differential response of grapevine to infection with ʼCandidatus Phytoplasma solaniʼ in early and late growing season through complex regulation of mRNA and small RNA transcriptomes

Dermastia, Marina (author), Škrlj, Blaž (author), Strah, Rebeka (author), Anžič, Barbara (author), Tomaž, Špela (author), Križnik, Maja (author), Schönhuber, Christina (author), Riedle-Bauer, Monika (author), Ramšak, Živa (author), Petek, Marko (author), Kladnik, Aleš (author), Lavrač, Nada (author), Gruden, Kristina (author), Roitsch, Thomas (author), Brader, Günter (author), Pompe Novak, Maruša (author)

Bois noir is the most widespread phytoplasma grapevine disease in Europe. It is associated with ‘Candidatus Phytoplasma solani’, but molecular interactions between the causal pathogen and its host plant are not well understood. In this work, we combined the analysis of high-throughput RNA-Seq and sRNA-Seq data with interaction network analysis for finding new cross-talks among pathways involved in infection of grapevine cv. Zweigelt with ‘Ca. P. solani’ in early and late growing seasons. While the early growing season was very dynamic at the transcriptional level in asymptomatic grapevines, the regulation at the level of small RNAs was more pronounced later in the season when symptoms developed in infected grapevines. Most differentially expressed small RNAs were associated with biotic stress. Our study also exposes the less-studied role of hormones in disease development and shows that hormonal balance was already perturbed before symptoms development in infected grapevines. Analysis at the level of communities of genes and mRNAmicroRNA interaction networks revealed several new genes (e.g., expansins and cryptdin) that have not been associated with phytoplasma pathogenicity previously. These novel actors may present a new reference framework for research and diagnostics of phytoplasma diseases of grapevine.

• Language: English
• Keywords: ‘Candidatus Phytoplasma solani’, grapevine, bois noir, RNA-Seq, sRNA-Seq, miRNA, phasiRNA, hormones, interaction network
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 28 str.
• Numbering: Vol. 22, iss. 7, art. 3531
• PID: 20.500.12556/RUL-135200
• UDC: 577
• ISSN on article: 1661-6596
• DOI: 10.3390/ijms22073531
• COBISS.SI-ID: 58895619
• Publication date in RUL: 28.02.2022

Record is a part of a journal

• Title: International journal of molecular sciences
• Shortened title: Int. j. mol. sci.
• Publisher: MDPI
• ISSN: 1661-6596
• COBISS.SI-ID: 36217605

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.04.2021

Secondary language

• Language: Slovenian
• Keywords: ʼCandidatus Phytoplasma solaniʼ, vinska trta, počrnelost lesa, RNA-Seq, sRNA-Seq, miRNA, phasiRNA, hormoni, interakcijska omrežja