Germinated buckwheat : effects of dehulling on phenolics profile and antioxidant activity of buckwheat seeds

Živković, Andrej (author), Polak, Tomaž (author), Cigić, Blaž (author), Požrl, Tomaž (author)

The aim was to investigate the effects of the cold dehulling of buckwheat seeds on their germination, total phenolic content (TPC), antioxidant activity (AA) and phenolics composition. Cold dehulling had no negative effects on germination rate and resulted in faster rootlet growth compared to hulled seeds. Although the dehulling of the seeds significantly decreased TPC and AA, the germination of dehulled seeds resulted in 1.8-fold and 1.9-fold higher TPC and AA compared to hulled seeds. Liquid chromatography coupled to mass spectrometry identified several phenolic compounds in free and bound forms. Rutin was the major compound in hulled seeds (98 µg/g dry weight), orientin and vitexin in 96-h germinated dehulled seeds (2205, 1869 µg/g dry weight, respectively). During germination, the increases in the major phenolic compounds were around two orders of magnitude, which were greater than the increases for TPC and AA. As well as orientin and vitexin, high levels of other phenolic compounds were detected for dehulled germinated seeds (e.g., isoorientin, rutin; 1402, 967 µg/g dry weight, respectively). These data show that dehulled germinated seeds of buckwheat have great potential for use in functional foods as a dietary source of phenolic compounds with health benefits.

• Language: English
• Keywords: buckwheat, dehulling, germination, LC-MS, free phenolic, bound phenolic, antioxidant activity
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 16 str.
• Numbering: Vol. 10, iss. 4, art. 740
• PID: 20.500.12556/RUL-135193
• UDC: 633.12:581.142:547.56
• ISSN on article: 2304-8158
• DOI: 10.3390/foods10040740
• COBISS.SI-ID: 58392323
• Publication date in RUL: 28.02.2022

Record is a part of a journal

• Title: Foods
• Shortened title: Foods
• Publisher: MDPI
• ISSN: 2304-8158
• COBISS.SI-ID: 512252472

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.04.2021

Secondary language

• Language: Slovenian
• Keywords: ajda, Fagopyrum esculentum, semena, kalitev, luščenje, fenolne spojine, antioksidativna aktivnost

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0234
• Name: Integrirano živilstvo in prehrana