20.500.12556/RUL-135193 Germinated buckwheat effects of dehulling on phenolics profile and antioxidant activity of buckwheat seeds The aim was to investigate the effects of the cold dehulling of buckwheat seeds on their germination, total phenolic content (TPC), antioxidant activity (AA) and phenolics composition. Cold dehulling had no negative effects on germination rate and resulted in faster rootlet growth compared to hulled seeds. Although the dehulling of the seeds significantly decreased TPC and AA, the germination of dehulled seeds resulted in 1.8-fold and 1.9-fold higher TPC and AA compared to hulled seeds. Liquid chromatography coupled to mass spectrometry identified several phenolic compounds in free and bound forms. Rutin was the major compound in hulled seeds (98 µg/g dry weight), orientin and vitexin in 96-h germinated dehulled seeds (2205, 1869 µg/g dry weight, respectively). During germination, the increases in the major phenolic compounds were around two orders of magnitude, which were greater than the increases for TPC and AA. As well as orientin and vitexin, high levels of other phenolic compounds were detected for dehulled germinated seeds (e.g., isoorientin, rutin; 1402, 967 µg/g dry weight, respectively). These data show that dehulled germinated seeds of buckwheat have great potential for use in functional foods as a dietary source of phenolic compounds with health benefits. buckwheat dehulling germination LC-MS free phenolic bound phenolic antioxidant activity ajda Fagopyrum esculentum semena kalitev luščenje fenolne spojine antioksidativna aktivnost true false true Angleški jezik Slovenski jezik Članek v reviji 2022-02-28 12:00:56 2022-02-28 12:00:56 2022-09-14 04:12:05 0000-00-00 00:00:00 2021 0 0 16 str. iss. 4, art. 740 Vol. 10 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 633.12:581.142:547.56 2304-8158 10.3390/foods10040740 58392323 RAZ_Zivkovic_Andrej_2021.pdf RAZ_Zivkovic_Andrej_2021.pdf 1 791B5DD050D8946888DBEF56620050B1 7689ddb17521e0a20a0733f8a44fdfaba35fe3d5dc1d41a3f929e2ccfd69069b 3046e850-9886-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154094 https://www.mdpi.com/2304-8158/10/4/740 1 70ed95f5-9885-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154093 Biotehniška fakulteta 0 0 0