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Extracellular cystatin F is internalised by cytotoxic T lymphocytes and decreases their cytotoxicity
ID
Prunk, Mateja
(
Avtor
),
ID
Perišić, Milica
(
Avtor
),
ID
Jakoš, Tanja
(
Avtor
),
ID
Sabotič, Jerica
(
Avtor
),
ID
Švajger, Urban
(
Avtor
),
ID
Kos, Janko
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(3,56 MB)
MD5: B5FCEA90FFA7037B47AD076461BC3B0C
URL - Izvorni URL, za dostop obiščite
https://www.mdpi.com/2072-6694/12/12/3660
Galerija slik
Izvleček
Cystatin F is a protein inhibitor of cysteine cathepsins, peptidases involved in the activation of the effector molecules of the perforin/granzyme pathway. Cystatin F was previously shown to regulate natural killer cell cytotoxicity. Here, we show that extracellular cystatin F has a role in regulating the killing efficiency of cytotoxic T lymphocytes (CTLs). Extracellular cystatin F was internalised into TALL-104 cells, a cytotoxic T cell line, and decreased their cathepsin C and H activity. Correspondingly, granzyme A and B activity was also decreased and, most importantly, the killing efficiency of TALL-104 cells as well as primary human CTLs was reduced. The N-terminally truncated form of cystatin F, which can directly inhibit cathepsin C (unlike the full-length form), was more effective than the full-length inhibitor. Furthermore, cystatin F decreased cathepsin L activity, which, however, did not affect perforin processing. Cystatin F derived from K-562 target cells could also decrease the cytotoxicity of TALL-104 cells. These results clearly show that, by inhibiting cysteine cathepsin proteolytic activity, extracellular cystatin F can decrease the cytotoxicity of CTLs and thus compromise their function.
Jezik:
Angleški jezik
Ključne besede:
cystatin F
,
cathepsins
,
cytotoxic lymphocytes
,
granzymes
,
perforin
,
TALL-104
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
FFA - Fakulteta za farmacijo
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2020
Št. strani:
14 str.
Številčenje:
Vol. 12, iss. 12, art. 3660
PID:
20.500.12556/RUL-134854
UDK:
616.1
ISSN pri članku:
2072-6694
DOI:
10.3390/cancers12123660
COBISS.SI-ID:
43955203
Datum objave v RUL:
04.02.2022
Število ogledov:
1039
Število prenosov:
136
Metapodatki:
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Objavi na:
Gradivo je del revije
Naslov:
Cancers
Skrajšan naslov:
Cancers
Založnik:
MDPI
ISSN:
2072-6694
COBISS.SI-ID:
517914137
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:
06.12.2020
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
cistatin F
,
katepsini
,
citotoksični limfociti
Projekti
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
J4-1776
Naslov:
Izboljšanje imunoterapevtske vrednosti NK celic z modulacijo cistatina F
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P4-0127
Naslov:
Farmacevtska biotehnologija: znanost za zdravje
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