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Validation of medicinal leeches (Hirudo medicinalis) as a non-invasive blood sampling tool for hematology and biochemistry profiling in mammals
ID
Kvapil, Pavel
(
Avtor
),
ID
Tomášek, Oldřich
(
Avtor
),
ID
Bártová, Eva
(
Avtor
),
ID
Harej, Mojca
(
Avtor
),
ID
Kastelic, Marjan
(
Avtor
),
ID
Primožič, Tit
(
Avtor
),
ID
Kašpárková, Nikola
(
Avtor
),
ID
Račnik, Joško
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(581,10 KB)
MD5: 8DBF6D7416EF3D7FCEE0AA36739CB018
URL - Izvorni URL, za dostop obiščite
https://www.frontiersin.org/articles/10.3389/fvets.2022.831836/full
Galerija slik
Izvleček
Blood sampling is a challenging procedure in many captive animals. Although manual restraint or anesthesia are usually possible, they entail intense stress and a high risk of injuries or organ failure. Blood sampling using medicinal leeches (Hirudo medicinalis) represents a promising non-invasive alternative to venipuncture; however, leech blood meal was to date used only for qualitative analyses such as genetic or serological screenings. Hence, the aim of this study was to evaluate the suitability of the leech blood sampling method for quantification of hematological and biochemical parameters. Medicinal leeches were manually applied on 67 zoo animals of eleven species, and control blood samples were obtained by venipuncture of the jugular vein. The leeches drew up to 20ml of blood in 20 to 55min. Although most hematological and biochemical parameters were significantly altered in leech-derived samples, their values showed strong (r = 0.62–0.79; 10/24 parameters) to very strong (r > 0.8; 13/24 parameters) correlations with venipuncture in all blood parameters, except for sodium (r = 0.39). As the parameter alterations and correlations were similar among species, simple crossspecies regression formulas were sufficient to correct the alterations, thereby ensuring good repeatability between leeches and venipuncture in most parameters. Our data thus suggest that medicinal leeches can be used as a reliable non-invasive and stressreducing alternative to standard venipuncture, even for quantitative assays. This opens new opportunities for a significant improvement to animal welfare in zoological gardens, conservation programmes, and ecophysiological research, where quantification of blood parameters is often needed.
Jezik:
Angleški jezik
Ključne besede:
hematology
,
biochemistry
,
non-invasive blood sampling
,
medicinal leech
,
Hirudo medicinalis
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
VF - Veterinarska fakulteta
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2022
Št. strani:
11 str.
Številčenje:
Vol. 9, art. 831836
PID:
20.500.12556/RUL-134850
UDK:
636.09:616-07
ISSN pri članku:
2297-1769
DOI:
10.3389/fvets.2022.831836
COBISS.SI-ID:
96337667
Datum objave v RUL:
04.02.2022
Število ogledov:
712
Število prenosov:
139
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Objavi na:
Gradivo je del revije
Naslov:
Frontiers in veterinary science
Skrajšan naslov:
Front. vet. sci.
Založnik:
Frontiers Media S.A.
ISSN:
2297-1769
COBISS.SI-ID:
3969402
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:
04.02.2022
Projekti
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
University of Veterinary Science Brno
Številka projekta:
FVHE/Literák/ITA2020
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
Czech Science Foundation
Številka projekta:
GA21-22160S to OT
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