Simplified LC-MS method for analysis of sterols in biological samples

Skubic, Cene (avtor), Vovk, Irena (avtor), Rozman, Damjana (avtor), Križman, Mitja (avtor)

We developed a simple and robust liquid chromatographic/mass spectrometric method (LC-MS) for the quantitative analysis of 10 sterols from the late part of cholesterol synthesis (zymosterol, dehydrolathosterol, 7-dehydrodesmosterol, desmosterol, zymostenol, lathosterol, FFMAS, TMAS, lanosterol, and dihydrolanosterol) from cultured human hepatocytes in a single chromatographic run using a pentafluorophenyl (PFP) stationary phase. The method also avails on a minimized sample preparation procedure in order to obtain a relatively high sample throughput. The method was validated on 10 sterol standards that were detected in a single chromatographic LC-MS run without derivatization. Our developed method can be used in research or clinical applications for disease-related detection of accumulated cholesterol intermediates. Disorders in the late part of cholesterol synthesis lead to severe malformation in human patients. The developed method enables a simple, sensitive, and fast quantification of sterols, without the need of extended knowledge of the LC-MS technique, and represents a new analytical tool in the rising field of cholesterolomics.

• Jezik: Angleški jezik
• Ključne besede: cholesterol, cholesterol synthesis, sterol intermediates, LC-MS, pentafluorophenyl, PFP
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2020
• Št. strani: 10 str.
• Številčenje: Vol. 25, iss. 18, art. 4116
• PID: 20.500.12556/RUL-134649
• UDK: 616.1
• ISSN pri članku: 1420-3049
• DOI: 10.3390/molecules25184116
• COBISS.SI-ID: 28345859
• Datum objave v RUL: 24.01.2022

Gradivo je del revije

• Naslov: Molecules
• Skrajšan naslov: Molecules
• Založnik: MDPI
• ISSN: 1420-3049
• COBISS.SI-ID: 18462981

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 09.09.2020

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: holesterol, sinteza holesterola, sterolni intermediati