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Electroporation as an efficacy potentiator for antibiotics with different target sites
ID Lovšin, Žana (Avtor), ID Klančnik, Anja (Avtor), ID Kotnik, Tadej (Avtor)

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Izvleček
Antibiotic resistance is a global health threat, and there is ample motivation for development of novel antibacterial approaches combining multiple strategies. Electroporation is among the promising complementary techniques – highly optimizable, effective against a broad range of bacteria, and largely impervious to development of resistance. To date, most studies investigating electroporation as an efficacy potentiator for antibacterials used substances permissible in food industry, and only few used clinical antibiotics, as acceptable applications are largely limited to treatment of wastewaters inherently contaminated with such antibiotics. Moreover, most studies have focused mainly on maximal achievable effect, and less on underlying mechanisms. Here, we compare Escherichia coli inactivation potentiation rates for three antibiotics with different modes of action: ampicillin (inhibits cell wall synthesis), ciprofloxacin (inhibits DNA replication), and tetracycline (inhibits protein synthesis). We used concentrations for each antibiotic from 0 to 30× its minimum inhibitory concentration, a single 1-ms electric pulse with amplitude from 0 to 20 kV/cm, and post-pulse pre-dilution incubation either absent (≲1 min) or lasting 60 min, 160 min, or 24 h. Our data show that with incubation, potentiation is significant for all three antibiotics, increases consistently with pulse amplitude, and generally also with antibiotic concentration and incubation time. With incubation, potentiation for ampicillin was rather consistently (although with weak statistical significance) superior to both ciprofloxacin and tetracycline: ampicillin was superior to both in 42 of 48 data points, including 7 with significance with respect to both, while at 60- and 160-min incubation, it was superior in 31 of 32 data points, including 6 with significance with respect to both. This suggests that electroporation potentiates wall-targeting antibiotics more than those with intracellular targets, providing motivation for in-depth studies of the relationship between the mode of action of an antibiotic and its potentiation by electroporation. Identification of substances permissible in foods and targeting the cell wall of both Gram-negative and Gram-positive bacteria might provide candidate antibacterials for broad and strong potentiation by electroporation applicable also for food preservation.

Jezik:Angleški jezik
Ključne besede:electroporation, antibiotics, mode of action, combined antibacterial treatments, wastewater treatment, treatment time
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:BF - Biotehniška fakulteta
FE - Fakulteta za elektrotehniko
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2021
Št. strani:11 str.
Številčenje:Vol. 12, art. 722232
PID:20.500.12556/RUL-134193 Povezava se odpre v novem oknu
UDK:602.621
ISSN pri članku:1664-302X
DOI:10.3389/fmicb.2021.722232 Povezava se odpre v novem oknu
COBISS.SI-ID:79973891 Povezava se odpre v novem oknu
Datum objave v RUL:28.12.2021
Število ogledov:449
Število prenosov:73
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Gradivo je del revije

Naslov:Frontiers in microbiology
Skrajšan naslov:Front. microbiol.
Založnik:Frontiers Media
ISSN:1664-302X
COBISS.SI-ID:4146296 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:18.10.2021

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:elektroporacija, antibiotiki, način delovanja, kombiniranje protibakterijske strategije, protibakterijska obdelava odpadnih voda, čas obdelave

Projekti

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P2-0249
Naslov:Elektroporacija v biologiji, biotehnologiji in medicini

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Program financ.:Young researchers

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:I0-0022
Naslov:Mreža raziskovalnih infrastrukturnih centrov Univerze v Ljubljani (MRIC UL)

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