Analysis of miR-9-5p, miR-124-3p, miR-21-5p, miR-138-5p, and miR-1-3p in glioblastoma cell lines and extracellular vesicles

Zottel, Alja (avtor), Šamec, Neja (avtor), Kump, Ana (avtor), Raspor Dall'Olio, Lucija (avtor), Pužar Dominkuš, Pia (avtor), Romih, Rok (avtor), Hudoklin, Samo (avtor), Mlakar, Jernej (avtor), Nikitin, Daniil (avtor), Sorokin, Maxim (avtor), Buzdin, Anton A. (avtor), Jovchevska, Ivana (avtor), Komel, Radovan (avtor)

Glioblastoma (GBM), the most common primary brain tumor, is a complex and extremely aggressive disease. Despite recent advances in molecular biology, there is a lack of biomarkers, which would improve GBM's diagnosis, prognosis, and therapy. Here, we analyzed by qPCR the expression levels of a set of miRNAs in GBM and lower-grade glioma human tissue samples and performed a survival analysis in silico. We then determined the expression of same miRNAs and their selected target mRNAs in small extracellular vesicles (sEVs) of GBM cell lines. We showed that the expression of miR-21-5p was significantly increased in GBM tissue compared to lower-grade glioma and reference brain tissue, while miR-124-3p and miR-138-5p were overexpressed in reference brain tissue compared to GBM. We also demonstrated that miR-9-5p and miR-124-3p were overexpressed in the sEVs of GBM stem cell lines (NCH421k or NCH644, respectively) compared to the sEVs of all other GBM cell lines and astrocytes. VIM mRNA, a target of miR-124-3p and miR-138-5p, was overexpressed in the sEVs of U251 and U87 GBM cell lines compared to the sEVs of GBM stem cell line and also astrocytes. Our results suggest VIM mRNA, miR-9-5p miRNA, and miR-124-3p miRNA could serve as biomarkers of the sEVs of GBM cells.

• Jezik: Angleški jezik
• Ključne besede: glioblastoma, biomarkers, exosomes, small extracellular vesicles, miRNA, miR-9-5p, miR-21-5p, miR-124-3p, miR-138-5p, vimentin
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2020
• Št. strani: 22 str.
• Številčenje: Vol. 21, iss. 22, art. 8491
• PID: 20.500.12556/RUL-133849
• UDK: 616-006
• ISSN pri članku: 1422-0067
• DOI: 10.3390/ijms21228491
• COBISS.SI-ID: 36976899
• Datum objave v RUL: 17.12.2021

Gradivo je del revije

• Naslov: International journal of molecular sciences
• Skrajšan naslov: Int. j. mol. sci.
• Založnik: MDPI
• ISSN: 1422-0067
• COBISS.SI-ID: 2779162

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 11.11.2020

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: glioblastom, biomarkerji, eksosomi