Transporters and efflux pumps are the main mechanisms involved in Staphylococcus epidermidis adaptation and tolerance to didecyldimethylammonium chloride

Ribič, Urška (avtor), Jakše, Jernej (avtor), Toplak, Nataša (avtor), Koren, Simon (avtor), Kovač, Minka (avtor), Klančnik, Anja (avtor), Jeršek, Barbara (avtor)

Staphylococcus epidermidis cleanroom strains are often exposed to sub-inhibitory concentrations of disinfectants, including didecyldimethylammonium chloride (DDAC). Consequently, they can adapt or even become tolerant to them. RNA-sequencing was used to investigate adaptation and tolerance mechanisms of S. epidermidis cleanroom strains (SE11, SE18), with S. epidermidis SE11Ad adapted and S. epidermidis SE18To tolerant to DDAC. Adaptation to DDAC was identified with up-regulation of genes mainly involved in transport (thioredoxin reductase [pstS], the arsenic efflux pump [gene ID, SE0334], sugar phosphate antiporter [uhpT]), while down-regulation was seen for the Agr system (agrA, arC, agrD, psm, SE1543), for enhanced biofilm formation. Tolerance to DDAC revealed the up-regulation of genes associated with transporters (L-cysteine transport [tcyB]; uracil permease [SE0875]; multidrug transporter [lmrP]; arsenic efflux pump [arsB]); the down-regulation of genes involved in amino-acid biosynthesis (lysine [dapE]; histidine [hisA]; methionine [metC]), and an enzyme involved in peptidoglycan, and therefore cell wall modifications (alanine racemase [SE1079]). We show for the first time the differentially expressed genes in DDAC-adapted and DDAC-tolerant S. epidermidis strains, which highlight the complexity of the responses through the involvement of different mechanisms.

• Jezik: Angleški jezik
• Ključne besede: Staphylococcus epidermidis, RNA-seq, didecyldimethylammonium chloride, adaptation mechanisms, tolerance mechanisms
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2020
• Št. strani: 18 str.
• Številčenje: Vol. 8, iss. 3, art. 344
• PID: 20.500.12556/RUL-133620
• UDK: 579.24+579.26:613.6
• ISSN pri članku: 2076-2607
• DOI: 10.3390/microorganisms8030344
• COBISS.SI-ID: 5173368
• Datum objave v RUL: 06.12.2021

Gradivo je del revije

• Naslov: Microorganisms
• Skrajšan naslov: Microorganisms
• Založnik: MDPI AG
• ISSN: 2076-2607
• COBISS.SI-ID: 523277081

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 01.03.2020

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: mikrobiološka kontrola obratov, čisti prostori, patogeni mikroorganizmi, Staphylococcus epidermidis, razkuževanje, didecildimetilamonijev klorid, DDAC, sekvencioniranje RNA, RNA-seq, biofilmi, izlivne črpalke, mehanizmi prilagoditve

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P4-0116
• Naslov: Mikrobiologija in biotehnologija živil in okolja

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: J4-9299
• Naslov: Mehanizmi adhezije bakterij Campylobacter kot tarča za zmanjšanje antibiotske odpornosti