izpis_h1_title_alt

Dissection of dynamic transcriptome landscape of leaf, bract, and lupulin gland in hop (Humulus lupulus L.)
ID Kumar Mishra, Ajay (Avtor), ID Kocábek, Tomáš (Avtor), ID Nath, Vishnu Sukumari (Avtor), ID Awasthi, Praveen (Avtor), ID Shrestha, Ankita (Avtor), ID Kumar Killi, Uday (Avtor), ID Jakše, Jernej (Avtor), ID Patzak, Josef (Avtor), ID Krofta, Karel (Avtor), ID Matoušek, Jaroslav (Avtor)

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Izvleček
The hop plant (Humulus lupulus L.) produces several valuable secondary metabolites, such as prenylflavonoid, bitter acids, and essential oils. These compounds are biosynthesized in glandular trichomes (lupulin glands) endowed with pharmacological properties and widely implicated in the beer brewing industry. The present study is an attempt to generate exhaustive information of transcriptome dynamics and gene regulatory mechanisms involved in biosynthesis and regulation of these compounds, developmental changes including trichome development at three development stages, namely leaf, bract, and mature lupulin glands. Using high-throughput RNA-Seq technology, a total of 61.13, 50.01, and 20.18 Mb clean reads in the leaf, bract, and lupulin gland libraries, respectively, were obtained and assembled into 43,550 unigenes. The putative functions were assigned to 30,996 transcripts (71.17%) based on basic local alignment search tool similarity searches against public sequence databases, including GO, KEGG, NR, and COG families, which indicated that genes are principally involved in fundamental cellular and molecular functions, and biosynthesis of secondary metabolites. The expression levels of all unigenes were analyzed in leaf, bract, and lupulin glands tissues of hop. The expression profile of transcript encoding enzymes of BCAA metabolism, MEP, and shikimate pathway was most up-regulated in lupulin glands compared with leaves and bracts. Similarly, the expression levels of the transcription factors and structural genes that directly encode enzymes involved in xanthohumol, bitter acids, and terpenoids biosynthesis pathway were found to be significantly enhanced in lupulin glands, suggesting that production of these metabolites increases after the leaf development. In addition, numerous genes involved in primary metabolism, lipid metabolism, photosynthesis, generation of precursor metabolites/energy, protein modification, transporter activity, and cell wall component biogenesis were differentially regulated in three developmental stages, suggesting their involvement in the dynamics of the lupulin gland development. The identification of differentially regulated trichome-related genes provided a new foundation for molecular research on trichome development and differentiation in hop. In conclusion, the reported results provide directions for future functional genomics studies for genetic engineering or molecular breeding for augmentation of secondary metabolite content in hop.

Jezik:Angleški jezik
Ključne besede:bitter acids, prenylflavonoids, terpenoids, trichome, Humulus lupulus, lupulin glands, RNA sequencing
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:BF - Biotehniška fakulteta
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2020
Št. strani:24 str.
Številčenje:Vol. 21, iss. 1, art. 233
PID:20.500.12556/RUL-133282 Povezava se odpre v novem oknu
UDK:577.2
ISSN pri članku:1422-0067
DOI:10.3390/ijms21010233 Povezava se odpre v novem oknu
COBISS.SI-ID:9402745 Povezava se odpre v novem oknu
Datum objave v RUL:19.11.2021
Število ogledov:820
Število prenosov:136
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
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Gradivo je del revije

Naslov:International journal of molecular sciences
Skrajšan naslov:Int. j. mol. sci.
Založnik:MDPI
ISSN:1422-0067
COBISS.SI-ID:2779162 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:01.01.2020

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:hmelj, genetika, RNA sekvenciranje, sekundarni metaboliti, molekularna biologija

Projekti

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Czech Science Foundation GACR
Številka projekta:19-19629S

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Operational Programme Research, Development and Education
Številka projekta:CZ.02.2.69/0.0/0.0/16_027/0008357
Akronim:MEMOBIC

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