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Geographical variability affects CCHFV detection by RT-PCR : a tool for in-silico evaluation of molecular assays
ID
Gruber, Cesare E. M.
(
Avtor
),
ID
Bartolini, Barbara
(
Avtor
),
ID
Castilletti, Concetta
(
Avtor
),
ID
Mirazimi, Ali
(
Avtor
),
ID
Hewson, Roger
(
Avtor
),
ID
Christova, Iva
(
Avtor
),
ID
Avšič-Županc, Tatjana
(
Avtor
),
ID
Grunow, Roland
(
Avtor
),
ID
Papa, Anna
(
Avtor
),
ID
Sánchez-Seco, María P.
(
Avtor
),
ID
Koopmans, Marion
(
Avtor
),
ID
Ippolito, Giuseppe
(
Avtor
),
ID
Capobianchi, Maria Rosaria
(
Avtor
),
ID
Reusken, Chantal B. E. M.
(
Avtor
),
ID
Di Caro, Antonino
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(692,78 KB)
MD5: 729D1DAE0D60D051544084789E1BE778
URL - Izvorni URL, za dostop obiščite
https://www.mdpi.com/1999-4915/11/10/953
Galerija slik
Izvleček
The Crimean-Congo hemorrhagic fever virus (CCHFV) is considered to be a major emerging infectious threat, according to the WHO R&D blueprint. A wide range of CCHFV molecular assays have been developed, employing varied primer/probe combinations. The high genetic variability of CCHFV often hampers the efficacy of available molecular tests and can affect their diagnostic potential. Recently, increasing numbers of complete CCHFV genomic sequences have become available, allowing a better appreciation of the genomic evolution of this virus. We summarized the current knowledge on molecular methods and developed a new bioinformatics tool to evaluate the existing assays for CCHFV detection, with a special focus on strains circulating in different geographical areas. Twenty-two molecular methods and 181 sequences of CCHFV were collected, respectively, from PubMed and GenBank databases. Up to 28 mismatches between primers and probes of each assay and CCHFV strains were detected through in-silico PCR analysis. Combinations of up to three molecular methods markedly decreased the number of mismatches within most geographic areas. These results supported the good practice of CCHFV detection of performing more than one assay, aimed for different sequence targets. The choice of the most appropriate tests must take into account patient's travel history and geographic distribution of the different CCHFV strains.
Jezik:
Angleški jezik
Ključne besede:
Crimean-Congo hemorrhagic fever
,
molecular detection
,
CCHFV
,
Crimean–Congo hemorrhagic fever virus
,
arthropod-borne virus
,
laboratory preparedness
,
emerging diseases
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
MF - Medicinska fakulteta
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2019
Št. strani:
14 str.
Številčenje:
Vol. 11, iss. 10, art. 953
PID:
20.500.12556/RUL-133136
UDK:
616.9
ISSN pri članku:
1999-4915
DOI:
10.3390/v11100953
COBISS.SI-ID:
34533081
Datum objave v RUL:
12.11.2021
Število ogledov:
1038
Število prenosov:
145
Metapodatki:
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Objavi na:
Gradivo je del revije
Naslov:
Viruses
Skrajšan naslov:
Viruses
Založnik:
MDPI
ISSN:
1999-4915
COBISS.SI-ID:
517597977
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:
16.10.2019
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
krimsko-kongoška hemoragična mrzlica
,
molekularno odkrivanje
,
arbovirus
Projekti
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
Italian Ministry of Health, Ricerca Corrente–Linea 1
Financer:
EC - European Commission
Številka projekta:
677066
Naslov:
Efficient response to highly dangerous and emerging pathogens at EU level
Akronim:
EMERGE
Financer:
EC - European Commission
Program financ.:
EVD-LabNet
Številka projekta:
ECDC/2016/00
Financer:
EC - European Commission
Program financ.:
H2020
Številka projekta:
653316
Naslov:
European Virus Archive goes global
Akronim:
EVAg
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