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Clinical and haplotypic variability of Slovenian USH2A patients homozygous for the c. 11864G>A nonsense mutation
ID Zupan, Andrej (Avtor), ID Fakin, Ana (Avtor), ID Battelino, Saba (Avtor), ID Jarc-Vidmar, Martina (Avtor), ID Hawlina, Marko (Avtor), ID Bonnet, Crystel (Avtor), ID Petit, Christine (Avtor), ID Glavač, Damjan (Avtor)

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Izvleček
Purpose: to determine a detailed clinical and haplotypic variability of the Slovenian USH2A patients with homozygous c.11864G>A (p.Trp3955Ter) nonsense mutation and to develop sensitive, accurate and rapid screening test. Methods: Ten unrelated homozygous patients with detailed ophthalmological exam were included in our study. The High-Resolution Melting (HRM) method was developed for fast and reliable detection of the c.11864G>A mutation. Results: The c.11864G>A mutation represents the vast majority of pathogenic alleles in Slovenian USH2A-Usher syndrome population (84%). The median age of onset of nyctalopia was 16 years and all patients younger than 40 years had hyperautofluorescent rings on fundus autofluorescence imaging. The Kaplan Meier survival analysis showed a decline of central vision after the age of 40, with 50% patients reaching visual acuity (VA) ≤ 0.05 at the average age of 66 years visual field diameter less than 20° at the average age of 59 years. There was a relatively large phenotypic variability in the retinal and audiological phenotype. Analysis of the p.Trp3955Ter-homozygous patients revealed four different haplotypes, with the frequency of the most common haplotype ~65%. Disease severity did not correlate with the haplotype. Conclusions: According to the natural history of homozygous p.Trp3955Ter patients any therapy aimed to slow disease progression in these patients would be best started before the age of 40. Phenotypic variability suggests the presence of cis and/or trans factors outside the USH2A gene that are able to affect disease severity. High frequency of p.Trp3955Ter mutation in Slovenian USH2A gene pool appears to be initiated from different unrelated founders because of migrations from neighboring populations. The mutation on haplotype 2 seems to be the major founder allele.

Jezik:Angleški jezik
Ključne besede:USH2A, haplotypic variability, homozygous mutation, usher syndrome, founder effect, haplotype analysis, high resolution melting analysis
Vrsta gradiva:Članek v reviji (dk_c)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:MF - Medicinska fakulteta
Leto izida:2019
Status objave pri reviji:Objavljeno
Verzija članka:Založnikova različica članka
Št. strani:12 str.
Številčenje:Vol. 10, iss. 12, art. 1015
UDK:617.7
ISSN pri članku:2073-4425
DOI:10.3390/genes10121015 Povezava se odpre v novem oknu
COBISS.SI-ID:34616281 Povezava se odpre v novem oknu
Datum objave v RUL:11.11.2021
Število ogledov:142
Število prenosov:47
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
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Gradivo je del revije

Naslov:Genes
Skrajšan naslov:Genes
Založnik:MDPI
ISSN:2073-4425
COBISS.SI-ID:523100185 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:05.12.2019

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:USH2A, haplotipska spremenljivost, homozigotna mutacija

Gradivo je financirano iz projekta

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Številka projekta:P3-0054
Naslov:Patologija in molekularna genetika

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Številka projekta:P3-0333
Naslov:Očesne bolezni odraslih in otrok

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